Equine Research Institute, Japan Racing Association, Shimotsuke, Tochigi, Japan.
Division of Virology, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, Tokyo, Japan.
Arch Virol. 2019 Sep;164(9):2355-2358. doi: 10.1007/s00705-019-04328-4. Epub 2019 Jun 21.
Equine influenza virus is an important pathogen for the horse industry because of its economic impact, and vaccination is a key control measure. Our previous work suggested that a mutation at position 144 in the hemagglutinin of Florida sublineage clade 2 viruses reduces the cross-neutralizing activity of antiserum against a former vaccine strain. To confirm this suggestion, here, we generated viruses by reverse genetics. Antibody titers against the mutated viruses were one-tenth to one-sixteenth of those against the former vaccine strain. Our findings confirm that this single amino acid substitution reduces the cross-reactivity of antiserum against this former Japanese vaccine.
马流感病毒因其经济影响对马业而言是一种重要的病原体，而疫苗接种是一种关键的控制措施。我们之前的工作表明，在血凝素 144 位发生突变的佛罗里达亚系 2 分支病毒会降低针对前一种疫苗株的抗血清的交叉中和活性。为了证实这一推测，在此，我们通过反向遗传学生成了病毒。针对突变病毒的抗体滴度比针对前一种疫苗株的抗体滴度低十分之一至十六分之一。我们的研究结果证实，这种单一氨基酸取代降低了抗血清对这种前日本疫苗的交叉反应性。
