通过反向遗传学在鸡胚中产生的具有不同骨架的重组马流感病毒的生长特性。

Growth properties of recombinant equine influenza viruses with different backbones generated by reverse genetics in embryonated chicken eggs.

作者信息

Nemoto Manabu, Kawanishi Nanako, Kambayashi Yoshinori, Bannai Hiroshi, Yamanaka Takashi, Garvey Marie, Cullinane Ann, Yamayoshi Seiya, Kawaoka Yoshihiro, Tsujimura Koji

机构信息

Equine Research Institute, Japan Racing Association, Tochigi, Japan.

Virology Unit, Irish Equine Centre, Kildare, Ireland.

出版信息

Arch Virol. 2025 Jul 12;170(8):181. doi: 10.1007/s00705-025-06368-5.

DOI:10.1007/s00705-025-06368-5

PMID:40646296

Abstract

Reverse genetics (RG) technology is useful for quickly updating influenza vaccine strains. A high-yield backbone (i.e., six segments other than hemagglutinin and neuraminidase) derived from A/Puerto Rico/8/1934 (PR8) has been developed to improve the growth of avian and human influenza viruses. However, for equine influenza virus (EIV), an EIV-derived backbone may have better growth properties due to more-natural segment combinations. We compared the growth properties in eggs between the PR8 backbone and an EIV backbone from A/equine/Ibaraki/1/2007, a vaccine strain in Japan. The results showed that the PR8 backbone was propagated more efficiently and is therefore more suitable for EIV vaccine production.

摘要

反向遗传学（RG）技术有助于快速更新流感疫苗毒株。已开发出一种源自A/波多黎各/8/1934（PR8）的高产骨架（即血凝素和神经氨酸酶以外的六个基因片段），以提高禽流感病毒和人流感病毒的生长能力。然而，对于马流感病毒（EIV），由于基因片段组合更自然，源自EIV的骨架可能具有更好的生长特性。我们比较了PR8骨架与来自日本疫苗株A/马/茨城/1/2007的EIV骨架在鸡胚中的生长特性。结果表明，PR8骨架的繁殖效率更高，因此更适合用于EIV疫苗生产。