Envirotransgene® Bio-solutions Global, Chennai, India.
DTCG®, Chennai, India.
Methods Mol Biol. 2020;2125:205-208. doi: 10.1007/7651_2019_249.
Baculovirus expression vector system (BEVS) is an established technology for recombinant protein expression in insect cells. Further, BEVS-mediated gene transduction of mammalian cells (BacMam) is emerging as a technique for high level recombinant protein expression in mammalian cells. Here, we describe generic method in using BEVS as a BacMam for rapid recombinant protein expression in mammalian cells.
杆状病毒表达载体系统（BEVS）是一种在昆虫细胞中表达重组蛋白的成熟技术。此外，杆状病毒介导的哺乳动物细胞转导（BacMam）作为一种在哺乳动物细胞中高水平表达重组蛋白的技术也正在兴起。本文中，我们描述了使用 BEVS 作为 BacMam 在哺乳动物细胞中快速表达重组蛋白的通用方法。
