Chambers Adam C, Aksular Mine, Graves Leo P, Irons Sarah L, Possee Robert D, King Linda A
Oxford Expression Technologies Ltd, Gipsy Lane, Oxford, United Kingdom.
Department of Biological & Medical Sciences, Oxford Brookes University, Oxford, United Kingdom.
Curr Protoc Protein Sci. 2018 Feb 21;91:5.4.1-5.4.6. doi: 10.1002/cpps.47.
This unit provides information on the replication cycle of insect baculovirus to provide an understanding of how this virus has been adapted for use as an expression vector for recombinant proteins in insect cells. We provide an overview of the virus structure and its unique bi-phasic replication cycle, which has been exploited in developing the virus as an expression vector. We also review the development of the baculovirus expression vector system (BEVS), from the mid-1980s to the present day in which the BEVS is now an established tool for the production of a range of recombinant proteins and multi-protein complexes including virus-like particles. We describe advances made to the BEVS to allow the rapid and easy production of recombinant viruses and developments to improve protein yield. We finish by describing the application of recombinant BacMam as vectors for the delivery of genes into mammalian and human cells. © 2018 by John Wiley & Sons, Inc.
本单元提供有关昆虫杆状病毒复制周期的信息，以帮助理解这种病毒是如何被改造用作昆虫细胞中重组蛋白的表达载体的。我们概述了病毒结构及其独特的双相复制周期，该周期已被用于将病毒开发为表达载体。我们还回顾了杆状病毒表达载体系统（BEVS）从20世纪80年代中期到现在的发展历程，如今BEVS已成为生产一系列重组蛋白和多蛋白复合物（包括病毒样颗粒）的成熟工具。我们描述了为使BEVS能够快速简便地生产重组病毒所取得的进展以及为提高蛋白产量所做的改进。最后，我们描述了重组BacMam作为将基因导入哺乳动物和人类细胞的载体的应用。© 2018约翰威立父子公司版权所有
