Surgical Oncology, The Second Affiliated Hospital of Hainan Medical College, Haikou, Hainan Province, China.
Mol Genet Genomic Med. 2019 Aug;7(8):e778. doi: 10.1002/mgg3.778. Epub 2019 Jun 22.
MIR155HG plays an important role in malignant tumors, but it is rarely reported in the occurrence and development of colorectal cancer (CRC). This study investigated the effects of MIR155HG polymorphisms on CRC susceptibility from the perspective of molecular genetics.
Eight SNPs in MIR155HG were selected and genotyped among 514 CRC cases and 510 healthy controls using the Agena MassARRAY platform. The associations between these SNPs and the CRC risk were evaluated under genetic models using conditional logistic regression analysis. The HaploReg v4.1 database was used for SNPs functional prediction.
The allele "C" of rs12482371 (p = 0.047), allele "C" of rs1893650 (p = 0.025), and the allele "A" of rs928883 (p = 0.037) in MIR155HG were significantly associated with CRC risk. Genetic model analysis revealed that rs12482371 and rs1893650 increased CRC risk; whereas rs928883 was associated with reduced CRC risk. Stratification analysis showed that rs9383938 was a protective factor in CRC patients under 60 years old. Rs12482371 and rs1893650 were associated with the CRC risk in females. Rs11911469 and rs34904192 may affect the clinical stage and lymph node metastasis. Moreover, the haplotypes CTT and GTC of LD block rs4143370|rs77218221|rs12482371, and the haplotypes CATGA and CACGG of LD block rs77699734|rs11911469|rs1893650|rs34904192|rs928883 were significantly associated with CRC risk.
This study revealed that MIR155HG SNPs were associated with CRC susceptibility and could be predictive biomarkers for CRC risk.
MIR155HG 在恶性肿瘤中发挥着重要作用，但在结直肠癌（CRC）的发生和发展中却鲜有报道。本研究从分子遗传学角度探讨了 MIR155HG 多态性对 CRC 易感性的影响。
在 514 例 CRC 病例和 510 例健康对照中，使用 Agena MassARRAY 平台选择 MIR155HG 中的 8 个 SNP 进行基因分型。采用条件逻辑回归分析，在遗传模型下评估这些 SNP 与 CRC 风险的相关性。使用 HaploReg v4.1 数据库对 SNP 功能进行预测。
MIR155HG 中的 rs12482371 （p=0.047）、rs1893650 （p=0.025）和 rs928883 （p=0.037）的等位基因“C”与 CRC 风险显著相关。遗传模型分析显示，rs12482371 和 rs1893650 增加了 CRC 风险；而 rs928883 与降低 CRC 风险相关。分层分析显示，rs9383938 是 60 岁以下 CRC 患者的保护因素。rs12482371 和 rs1893650 与女性 CRC 风险相关。rs11911469 和 rs34904192 可能影响临床分期和淋巴结转移。此外，LD 块 rs4143370|rs77218221|rs12482371 的 CTT 和 GTC 单体型以及 LD 块 rs77699734|rs11911469|rs1893650|rs34904192|rs928883 的 CATGA 和 CACGG 单体型与 CRC 风险显著相关。
本研究表明 MIR155HG SNP 与 CRC 易感性相关，可能是 CRC 风险的预测生物标志物。
