Lomako V V, Shylo O V
Institute for Problems of Cryobiology and Cryomedicine of NAS of Ukraine, 23 Pereyaslavskaya str., Kharkov 61016, Ukraine, e-mail:
Adv Gerontol. 2019;32(1-2):55-59.
The dynamics of transformation and osmotic fragility (OF) of erythrocytes in the blood of rats of different ages (6, 12 and 24 months) were studied using small-angle light scattering method and the indices of erythrocytes transformation were calculated. The heterogeneity of the erythrocytes population in the blood increased with age: the proportion of normal and highly resistant flattened discocytes decreased. The proportion of flattened discocytes was the lowest in 12 months of aged rats. Correspondently, the proportion of the erythrocytes altered shapes, both reversible (stomatocytes) and irreversible (spherocytes), increased with age. It is speculated that the erythrocytes transformation developed via the stomatocytic pathway. The tendency to an increase in the level of erythrocytes OF between the rat ages was only found. The indices of transformation, reversible and irreversible transformation increased with age, except for the reversibility index, which was the highest in 6 months of age rats, significantly unchanged in 12 months of age rats, and decreased almost in 3,5 times in 24 months compared to 6 months of aged rats. In 6 months` aged rats, a lower index of transformation was observed against a background of a higher index of reversibility compared to the other age groups of rats.
采用小角度光散射法研究了不同年龄（6、12和24个月）大鼠血液中红细胞的转化动力学和渗透脆性（OF），并计算了红细胞转化指标。血液中红细胞群体的异质性随年龄增加：正常和高抗性扁平盘状红细胞的比例下降。扁平盘状红细胞的比例在12月龄大鼠中最低。相应地，形状改变的红细胞比例，包括可逆性（口形红细胞）和不可逆性（球形红细胞），随年龄增加。推测红细胞转化是通过口形红细胞途径发生的。仅发现大鼠年龄之间红细胞OF水平有增加的趋势。转化指标、可逆和不可逆转化指标随年龄增加，除了可逆性指标，其在6月龄大鼠中最高，在12月龄大鼠中无显著变化，与6月龄大鼠相比，24月龄时几乎下降了3.5倍。与其他年龄组的大鼠相比，在6月龄大鼠中，在较高的可逆性指标背景下观察到较低的转化指标。
