Manni A, Badger B, Wright C, Ahmed S R, Santner S J, Luk G
Department of Medicine, Milton S. Hershey Medical Center, Pennsylvania State University, Hershey 17033-2396.
Breast Cancer Res Treat. 1987 Nov;10(2):191-6. doi: 10.1007/BF01810582.
The present experiments were designed to test whether polyamines play an essential role in the synthesis of growth factors induced by ovine prolactin (oPRL), using the N-nitrosomethylurea (NMU)-induced rat mammary tumor cultured in the soft agar clonogenic assay. Conditioned media (CM) obtained from tumors treated with oPRL and the polyamine biosynthesis inhibitor alpha-difluoromethylornithine (DFMO) (1 mM) no longer exerted the colony-stimulating effect which was observed with oPRL-CM. Such growth-promoting activity was restored with conditioned media obtained from tumors treated with oPRL, DFMO, and increasing concentrations of spermidine from 1 to 500 microM. The colony-stimulating effect of the CM employed could not be accounted for by the contaminating presence in the media of oPRL, DFMO, and polyamines. These results indicate that in our system polyamines play an important role in the synthesis of oPRL-regulated growth factors.
本实验旨在利用在软琼脂克隆形成试验中培养的N-亚硝基甲基脲（NMU）诱导的大鼠乳腺肿瘤，测试多胺在绵羊催乳素（oPRL）诱导的生长因子合成中是否起关键作用。用oPRL和多胺生物合成抑制剂α-二氟甲基鸟氨酸（DFMO）（1 mM）处理肿瘤后获得的条件培养基（CM）不再发挥oPRL-CM所观察到的集落刺激作用。用oPRL、DFMO和浓度从1至500 microM递增的亚精胺处理肿瘤后获得的条件培养基可恢复这种生长促进活性。所用CM的集落刺激作用不能用培养基中污染存在的oPRL、DFMO和多胺来解释。这些结果表明，在我们的系统中，多胺在oPRL调节的生长因子合成中起重要作用。
