Semeraro Paola, Syrgiannis Zois, Bettini Simona, Giancane Gabriele, Guerra Flora, Fraix Aurore, Bucci Cecilia, Sortino Salvatore, Prato Maurizio, Valli Ludovico
Department of Biological and Environmental Sciences and Technology (DiSTeBA), Campus University Ecotekne, University of Salento, Via per Monteroni, I-73100 Lecce, Italy.
Simpson Querrey Institute for BioNanotechnology, Northwestern University.
J Colloid Interface Sci. 2019 Oct 1;553:390-401. doi: 10.1016/j.jcis.2019.06.037. Epub 2019 Jun 12.
A perylene bisimide (PBI) derivative was utilized as photosensitizer for photodynamic therapy (PDT) applications, due to its high efficiency in singlet oxygen generation upon photoexcitation. It was immobilized onto a hydrophobized solid support, by means of the Langmuir-Schaefer (LS) technique, to achieve a preliminary medical device able to induce death of cancer cells in vitro. First, PBI derivative solutions, at two different concentrations (4.2 × 10 and 1.5 × 10 M) were chosen, based on the different PBI aggregation state, to be spread onto a water subphase in a Langmuir trough. Physico-chemical and morphological characterizations of the floating films were performed. Then the floating layers were transferred onto quartz substrates. The resulting multilayer LS films were characterized by spectroscopic measurements showing that the photochemical properties of the PBI derivative were well preserved even when immobilized. The LS film that exhibited the highest efficiency in the singlet oxygen production under light excitation was assessed in in vitro tests on human cervical carcinoma C13 cell line and the photo-toxicity was measured. This study revealed absence of cytotoxicity in dark conditions and a high photo-cytotoxicity toward cancer cells, making it a promising photoactive device.
由于其在光激发时产生单线态氧的高效率，苝二酰亚胺（PBI）衍生物被用作光动力疗法（PDT）应用的光敏剂。通过朗缪尔-谢弗（LS）技术将其固定在疏水化固体载体上，以获得一种能够在体外诱导癌细胞死亡的初步医疗设备。首先，基于不同的PBI聚集状态，选择两种不同浓度（4.2×10和1.5×10 M）的PBI衍生物溶液，将其铺展在朗缪尔槽中的水亚相上。对漂浮膜进行了物理化学和形态表征。然后将漂浮层转移到石英基板上。通过光谱测量对所得的多层LS膜进行了表征，结果表明即使固定后，PBI衍生物的光化学性质仍得到很好的保留。在对人宫颈癌C13细胞系的体外测试中评估了在光激发下产生单线态氧效率最高的LS膜，并测量了其光毒性。这项研究表明在黑暗条件下没有细胞毒性，并且对癌细胞具有高光细胞毒性，使其成为一种有前景的光活性设备。
