College of Food Science and Technology, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
School of Chemical Engineering, Northwest University, 229 North TaiBai Road, Xi'an 710069, China.
Food Res Int. 2019 Aug;122:241-251. doi: 10.1016/j.foodres.2019.04.022. Epub 2019 Apr 10.
Colitis is a major chronic disease in today's society. Consumption of phenolic compounds can protect against colitis. We examined the phenolic compounds of rape bee pollen extract (RPE) and investigated its prevention of DSS-induced colitis in C57BL/6 mice. HPLC-DAD-ECD analysis showed that 7 phenolic compounds were tentatively identified in RPE, of which kaempferol had the highest content (19.87 mg/g), followed by sinapic acid and rosmarinic acid. RPE at 21.2 g/kg BW (HD - high-dose group) and 10.6 g/kg BW (LD - low-dose group) ameliorated colon shortening, spleen swelling and colon weight reduction, improved the structure of colonic villi, glandular structures and crypts, which was further confirmed by conventional histological assessment, and inhibited the activities of related inflammatory cytokines. In particular, IL-1β expression was downregulated by approximately 90% compared with that of the model group. Additionally, treatment with RPE altered the gut microbial structure of mice with colitis, showing that the abundances of Allobaculum and Bacteroides were significantly reduced and the abundance of Lactobacillus was markedly increased. This study indicated that RPE could protect the colon from DSS-induced colitis by increasing antioxidant capacity, alleviating inflammation and regulating the gut microbiota. This paper was conducted to investigate the potent protective effects of RPE treatments on a mouse model of colitis and provided new ideas for the application of rape bee pollen.
结肠炎是当今社会的一种主要慢性疾病。消费酚类化合物可以预防结肠炎。我们检查了油菜蜂花粉提取物（RPE）中的酚类化合物，并研究了其对 C57BL/6 小鼠 DSS 诱导结肠炎的预防作用。HPLC-DAD-ECD 分析表明，RPE 中暂定鉴定出 7 种酚类化合物，其中山奈酚含量最高（19.87mg/g），其次是芥子酸和迷迭香酸。RPE 以 21.2g/kg BW（HD-高剂量组）和 10.6g/kg BW（LD-低剂量组）改善了结肠缩短、脾脏肿胀和结肠重量减轻，改善了结肠绒毛、腺体结构和隐窝的结构，这进一步通过常规组织学评估得到证实，并抑制了相关炎症细胞因子的活性。特别是，与模型组相比，IL-1β 的表达降低了约 90%。此外，RPE 处理改变了结肠炎小鼠的肠道微生物结构，表明 Allobaculum 和 Bacteroides 的丰度明显降低，Lactobacillus 的丰度显著增加。本研究表明，RPE 通过增加抗氧化能力、缓解炎症和调节肠道微生物群来保护结肠免受 DSS 诱导的结肠炎。本研究旨在探讨 RPE 治疗对结肠炎小鼠模型的潜在保护作用，为油菜蜂花粉的应用提供了新的思路。
