Hainan Provincial Engineering Research Centre of Aquatic Resources Efficient Utilization in the South China Sea, China.
Food Funct. 2020 Apr 30;11(4):3245-3255. doi: 10.1039/d0fo00116c.
In this study, we evaluated the effects of tilapia head glycolipids (TH-GLs) on male C57BL/6 mice with inflammatory bowel disease (IBD) induced by dextran sulfate sodium (DSS) and the changes in gut microbiota compared with sulfasalazine. Mice were orally treated with 3% (w/v) DSS or not for 7 days, followed by drug treatment with TH-GLs or sulfasalazine. After treatment, macroscopic colitis symptoms, intestinal epithelial barrier function, inflammatory cytokines, and gut microbiota homeostasis were assessed. Further studies showed that TH-GLs and sulfasalazine showed different influences on the gut microbiota structure. Both sulfasalazine and TH-GLs decreased the DSS-induced enrichment of Gammaproteobacteria and Enterobacteriaceae. However, TH-GLs had a selective increase in the enrichment of Akkermansia, Prevotellaceae, Oscillospira, Allobaculum, Bifidobacterium, and Coprococcus in contrast to sulfasalazine, which selectively increased the enrichment of Dorea, Turicibacter, Bacteroides, Coprobacillus, Mucispirillum, and Dehalobacterium. In addition, both TH-GLs and sulfasalazine relieved body weight loss, and increased the immune organ index, while maintaining the balance between pro-inflammatory and anti-inflammatory cytokines. The results indicate that TH-GLs alleviate DSS-induced IBD in mice by decreasing the abundance of harmful gut microbiota and enhancing the abundance of probiotic gut microbiota. Thus, the mechanism through which TH-GLs inhibit inflammation through gut microbiota is different from that of sulfasalazine. Therefore, TH-GLs stand as potential prebiotics for the treatment of colonic inflammation and related diseases.
在这项研究中，我们评估了罗非鱼头糖脂（TH-GLs）对葡聚糖硫酸钠（DSS）诱导的炎症性肠病（IBD）雄性 C57BL/6 小鼠的影响，并与柳氮磺胺吡啶（sulfasalazine）相比，观察肠道微生物群的变化。小鼠经口给予 3%（w/v）DSS 处理 7 天，然后给予 TH-GLs 或柳氮磺胺吡啶进行药物治疗。治疗后，评估宏观结肠炎症状、肠上皮屏障功能、炎症细胞因子和肠道微生物群稳态。进一步的研究表明，TH-GLs 和柳氮磺胺吡啶对肠道微生物群结构有不同的影响。柳氮磺胺吡啶和 TH-GLs 均降低了 DSS 诱导的γ变形菌门和肠杆菌科的富集。然而，与柳氮磺胺吡啶相比，TH-GLs 选择性地增加了阿克曼菌、普雷沃氏菌科、双歧杆菌、拟杆菌属、梭菌属和真杆菌属的富集，而柳氮磺胺吡啶选择性地增加了 Dorea、Turicibacter、拟杆菌属、梭菌属、穆氏螺旋菌和脱硫弧菌的富集。此外，TH-GLs 和柳氮磺胺吡啶均缓解了体重减轻，并增加了免疫器官指数，同时维持了促炎细胞因子和抗炎细胞因子之间的平衡。结果表明，TH-GLs 通过降低有害肠道微生物群的丰度和增强益生菌肠道微生物群的丰度来缓解 DSS 诱导的 IBD 小鼠模型。因此，TH-GLs 通过肠道微生物群抑制炎症的机制与柳氮磺胺吡啶不同。因此，TH-GLs 可能成为治疗结肠炎症和相关疾病的潜在益生元。
