Roy Sonti, Bhandari Vasundhra, Dandasena Debabrata, Murthy Shweta, Sharma Paresh
National Institute of Animal Biotechnology, Hyderabad, India.
Manipal Academy of Higher Education, Manipal, India.
Front Physiol. 2019 Jun 7;10:673. doi: 10.3389/fphys.2019.00673. eCollection 2019.
Tropical theileriosis caused by infection is a significant livestock disease affecting cattle health and productivity resulting in substantial monetary losses in several countries. Despite the use of an effective vaccine for disease control still, a high incidence of infection is reported from India. One of the many reasons behind the ineffective disease control can be the existence of genetically diverse parasite population in India. Therefore, studies focusing on understanding the genotypes are warranted. In this study, we have performed a genetic analysis of the Indian field cell lines and the vaccine line using microsatellite markers, Genotyping based sequencing (GBS) and gene polymorphism. The degree of allelic diversity and multiplicity of the infection was determined to be high in the Indian population. No geographical sub-structuring and linkage disequilibrium were observed in the population. High population diversity was found which were similar with countries like Oman, Tunisia, and Turkey in contrast to Portugal and China. The presence of multiple genotypes as determined by microsatellite marker genotyping, GBS analysis and gene polymorphism point toward a panmictic parasite population in India. These findings are the first report from India which would help in understanding the evolution and diversity of the population in the country and can help in designing more effective strategies for controlling the disease.
由感染引起的热带泰勒虫病是一种严重的家畜疾病，影响牛的健康和生产力，在多个国家造成了巨大的经济损失。尽管使用了有效的疫苗来控制疾病，但印度仍报告了高感染率。疾病控制无效的众多原因之一可能是印度存在基因多样化的寄生虫种群。因此，有必要开展侧重于了解基因型的研究。在本研究中，我们使用微卫星标记、基于基因分型的测序（GBS）和基因多态性对印度的田间细胞系和疫苗系进行了遗传分析。确定印度种群中感染的等位基因多样性程度和多重性较高。在该种群中未观察到地理亚结构和连锁不平衡。发现了较高的种群多样性，与阿曼、突尼斯和土耳其等国家相似，与葡萄牙和中国不同。通过微卫星标记基因分型、GBS分析和基因多态性确定存在多种基因型，这表明印度存在随机交配的寄生虫种群。这些发现是来自印度的首份报告，将有助于了解该国该种群的进化和多样性，并有助于设计更有效的疾病控制策略。
