Kong Qingxian, Guo Xing, Guo Zhirui, Su Tao
Clin Lab. 2019 Jun 1;65(6). doi: 10.7754/Clin.Lab.2018.180921.
Exosomes are potential markers for several diseases and health problems. Type 1 diabetes (T1D) has increasingly been reported in children and the world T1D has become the main type of diabetes in children. This study aimed to understand the function of urinary exosomal miRNAs in T1D in children.
We collected urinary samples from 30 healthy controls and 30 T1D in children. All exosomes were isolated with a combined centrifugation and were characterized by electron microscopy and western blot. The small RNA sequence was used to detect the urinary exosomal miRNAs, and miRNA markers were validated by real-time quantitative PCR. Two exosome diagnostic miRNAs were confirmed by receiver operating characteristic analyses.
In this study, we found higher urinary exosomal miR-424 and miR-218 expression in T1D in children than in healthy controls. Urinary exosomal miR-424 and miR-218 showed better accuracy for T1D diagnose in children.
Our results suggest that urinary exosomal miR-424 and miR-218 are biomarkers for T1D detection in children; miR-424 and miR-218 may be predictive of T1D prognosis in children.
外泌体是多种疾病和健康问题的潜在标志物。1型糖尿病（T1D）在儿童中的报道日益增多，且已成为全球儿童糖尿病的主要类型。本研究旨在了解儿童T1D中尿外泌体微小RNA（miRNA）的功能。
我们收集了30名健康对照儿童和30名T1D儿童的尿液样本。所有外泌体均通过联合离心法分离，并通过电子显微镜和蛋白质印迹法进行鉴定。利用小RNA测序检测尿外泌体miRNA，并通过实时定量聚合酶链反应验证miRNA标志物。通过受试者工作特征分析确定了两种外泌体诊断性miRNA。
在本研究中，我们发现儿童T1D患者尿外泌体miR - 424和miR - 218的表达高于健康对照儿童。尿外泌体miR - 424和miR - 218对儿童T1D的诊断具有更高的准确性。
我们的结果表明，尿外泌体miR - 424和miR - 218是儿童T1D检测的生物标志物；miR - 424和miR - 218可能预测儿童T1D的预后。
