Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India; Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi 110067, India.
Materials Research Laboratory, Department of Chemistry, Jamia Millia Islamia, New Delhi 110025, India; Advanced Instrumentation Research Facility, Jawaharlal Nehru University, New Delhi 110067, India.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Nov 5;222:117279. doi: 10.1016/j.saa.2019.117279. Epub 2019 Jun 16.
Imaging technologies play a vital role in clinical oncology and have undergone massive growth over the past few decades. Research in the field of tumor imaging and biomedical diagnostics requires early detection of physiological alterations so as to provide curative treatment in real time. The objective of this review is to provide an insight about near infrared fluorescence (NIRF) and surface enhanced Raman scattering (SERS) imaging techniques that can be used to expand their capabilities for the early detection and diagnosis of cancer cells. Basic setup, principle and working of the instruments has been provided and common NIRF imaging agents as well as SERS tags are also discussed besides the analytical advantages/disadvantages of these techniques. This review can help researchers working in the field of molecular imaging to design cost effective fluorophores and SERS tags to overcome the limitations of both NIRF as well as SERS imaging technologies.
成像技术在临床肿瘤学中起着至关重要的作用，在过去几十年中经历了飞速发展。肿瘤成像和生物医学诊断领域的研究需要早期检测生理变化，以便实时提供治疗。本篇综述的目的是提供关于近红外荧光（NIRF）和表面增强拉曼散射（SERS）成像技术的深入了解，这些技术可用于扩展其早期检测和诊断癌细胞的能力。提供了仪器的基本设置、原理和工作原理，并讨论了常见的 NIRF 成像剂和 SERS 标记物，以及这些技术的分析优缺点。本篇综述可以帮助从事分子成像研究的研究人员设计具有成本效益的荧光团和 SERS 标记物，以克服 NIRF 和 SERS 成像技术的局限性。
