Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand.
Center of Excellence in Bioresources for Agriculture, Industry and Medicine, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
Sci Rep. 2019 Jun 24;9(1):9161. doi: 10.1038/s41598-019-45647-5.
Sapria himalayana Griffith., is a root parasitic plant that is exceptionally beautiful and odd-looking and found in Southeast Asia. Now these plants are at risk of extinction as they face a large number of different threats. Appropriate measures and conservation plans are needed and one crucial key for successful conservation is species monitoring. The flower is the only part of S. himalayana that is visible during a short period of time of the year. Thus, conducting a visual survey in the field at the other times of the year would be difficult. DNA from living organisms could be found accumulating in environment and so-called environmental DNA (eDNA). Here, an eDNA-based method was developed to specifically monitor S. himalayana in nature. Detecting the specifically generated amplicons allowed us to monitor the presence of S. himalayana at any time of the year. This developed method would increase the conservation success of the S. himalayana.
喜马拉雅扁蕾（Sapria himalayana Griffith.）是一种根寄生植物，外形奇特而美丽，分布于东南亚地区。由于面临着多种威胁，这些植物现在正面临灭绝的危险。因此，需要采取适当的措施和保护计划，而物种监测是成功保护的关键之一。在一年中的短时间内，喜马拉雅扁蕾的花是唯一可见的部分。因此，在一年中的其他时间在野外进行目视调查将非常困难。生物体的 DNA 可以在环境中积累，这种所谓的环境 DNA（eDNA）。在这里，开发了一种基于 eDNA 的方法来专门监测自然中的喜马拉雅扁蕾。检测到特定生成的扩增子使我们能够随时监测喜马拉雅扁蕾的存在。这种开发的方法将提高喜马拉雅扁蕾的保护成功率。
