使用 DREAM 测定法筛选作为微生物燃料电池中的分析物的沉积物样品中的微生物电子传递活性。
Screening sediment samples used as anolytes in microbial fuel cells for microbial electron transfer activity using DREAM assay.
机构信息
Department of Biosciences, Sri Sathya Sai Institute of Higher Learning, Prasanthi Nilayam, Puttaparthi, Andhra Pradesh, 515134, India.
出版信息
Biotechnol Lett. 2019 Sep;41(8-9):979-985. doi: 10.1007/s10529-019-02704-3. Epub 2019 Jun 24.
OBJECTIVE
The dye reduction-based electron-transfer activity monitoring (DREAM) assay was employed to screen sediment and wastewater samples functioning as anolytes in a microbial fuel cell (MFC) for their microbial electron transfer activity.
RESULTS
Electron transfer to redox dyes from microbial activity reduced the dyes and the resulting extent of reduction was measured as DREAM assay coefficient. Methylene blue was decolourised, while resazurin underwent florigenic change from blue to pink to colourless upon formation of resorufin and dihydroxyresorufin. DREAM assay coefficient conformed to power density obtained in the MFC. A correlation was observed between chemical oxygen demand of the sample and the DREAM coefficient (+ 0.934) and also between DREAM coefficient and power density generated (+ 0.976). Highest DREAM coefficient and power density was observed for activated sludge.
CONCLUSIONS
The DREAM assay is a rapid, sensitive and low-cost method to assess microbial electron transfer activity for inocula used as anolytes in a MFC.
目的
采用基于染料还原的电子转移活性监测（DREAM）测定法筛选作为微生物燃料电池（MFC）阳极的沉积物和废水样品的微生物电子转移活性。
结果
微生物活性向氧化还原染料的电子转移使染料还原，还原的程度用 DREAM 测定系数来衡量。亚甲蓝被脱色，而 Resazurin 在形成 Resorufin 和 Dihydroxyresorufin 后，从蓝色变为粉红色再变为无色，发生了花色素原变化。DREAM 测定系数与 MFC 中获得的功率密度一致。观察到样品的化学需氧量与 DREAM 系数（+0.934）之间存在相关性，以及 DREAM 系数与产生的功率密度（+0.976）之间存在相关性。活性污泥的 DREAM 系数和功率密度最高。
结论
DREAM 测定法是一种快速、灵敏和低成本的方法，可用于评估用作 MFC 阳极的接种物的微生物电子转移活性。
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