Carter R A, Ericsson S A, Corn C D, Weyerts P R, Dart M G, Escue S G, Mesta J
Division of Range Animal Science, Sul Ross State University, Alpine, Texas 79832, USA.
Arch Androl. 1998 Jan-Feb;40(1):59-66. doi: 10.3109/01485019808987928.
The objective of this study was to determine if spermatozoal reduction of the dyes methylene green to colorless and resazurin to pink or colorless was associated with the fertility potential of an equine semen sample. Fifty samples from 38 stallions were evaluated for the number of spermatozoa per milliliter and number of motile sperm per milliliter. Methylene green (20 micrograms/mL of semen) or resazurin (85 micrograms/mL of semen) was added to 3-mL aliquots of semen. Semen samples were identified as having low fertility potential (< 200 x 10(6) total cells/mL and < 140 x 10(6) motile cells/mL) or high fertility potential (> or = 200 x 10(6) total cells/mL and > or = 140 x 10(6) motile cells/mL). The sensitivities were 80% for the methylene green, 68% for the resazurin to pink, and 60% for the resazurin to colorless tests. The specificities were 80% for the methylene green, 79% for the resazurin to pink, and 92% for the resazurin to colorless tests. The overall accuracies were 80% for the methylene green test, 74% for the resazurin to pink test, and 76% for the resazurin to colorless tests. The methylene green and resazurin reduction tests can provide valuable information on the fertility potential of an equine semen sample.
本研究的目的是确定精子将亚甲绿还原为无色以及将刃天青还原为粉红色或无色是否与马精液样本的生育潜力相关。对来自38匹种马的50份样本进行了每毫升精子数量和每毫升活动精子数量的评估。将亚甲绿（20微克/毫升精液）或刃天青（85微克/毫升精液）添加到3毫升精液等分试样中。精液样本被鉴定为生育潜力低（<200×10⁶个总细胞/毫升且<140×10⁶个活动细胞/毫升）或生育潜力高（≥200×10⁶个总细胞/毫升且≥140×10⁶个活动细胞/毫升）。亚甲绿试验的敏感性为80%，刃天青还原为粉红色试验的敏感性为68%，刃天青还原为无色试验的敏感性为60%。亚甲绿试验的特异性为80%，刃天青还原为粉红色试验的特异性为79%，刃天青还原为无色试验的特异性为92%。亚甲绿试验的总体准确率为80%，刃天青还原为粉红色试验的总体准确率为74%，刃天青还原为无色试验的总体准确率为76%。亚甲绿和刃天青还原试验可为马精液样本的生育潜力提供有价值的信息。
