巨大芽孢杆菌孢子及孢子膜的脂质组成

Lipid composition of Bacillus megaterium spores and spore membranes.

作者信息

Nikolopoulou M, Vary J C

机构信息

Department of Biological Chemistry, University of Illinois, Chicago 60612.

出版信息

Lipids. 1987 Oct;22(10):698-703. doi: 10.1007/BF02533968.

DOI:10.1007/BF02533968

PMID:3123837

Abstract

Bacillus megaterium QM B1551 spore lipids were extracted by an improved technique, and the phospholipid and fatty acid compositions were determined. Phospholipids accounted for 65% of the total fatty acids; the neutral lipid fraction contained 15% and the remaining fatty acids were in the interphase, aqueous phase and pellet from the lipid extraction. Each phospholipid had similar fatty acid compositions as did the delipidated pellet. However, the aqueous phase and, to some extent, the interphase had unique fatty acid compositions. Also, fatty acids were found acylated to proteins, which was observed by electrophoresis of delipidated proteins from spores grown in [1-14C]palmitate. Therefore, spores contain unique non-phosphatide fatty acid components that can now be analyzed.

摘要

巨大芽孢杆菌QM B1551的孢子脂质通过改进技术提取，并测定了磷脂和脂肪酸组成。磷脂占总脂肪酸的65%；中性脂质部分占15%，其余脂肪酸存在于脂质提取的中间相、水相和沉淀中。每种磷脂的脂肪酸组成与脱脂沉淀相似。然而，水相以及在一定程度上中间相具有独特的脂肪酸组成。此外，还发现脂肪酸酰化到蛋白质上，这是通过对在[1-14C]棕榈酸盐中生长的孢子的脱脂蛋白质进行电泳观察到的。因此，孢子含有独特的非磷脂脂肪酸成分，现在可以对其进行分析。