高眼压对大鼠视网膜中含D-天冬氨酸蛋白的影响。
Effect of Ocular Hypertension on D--Aspartic Acid-Containing Proteins in the Retinas of Rats.
作者信息
Kanamoto Takashi, Tachibana Takashi, Kitaoka Yasushi, Hisatomi Toshio, Ikeda Yasuhiro, Murakami Yusuke, Tobiume Kei, Asaoka Ryo, Kiuchi Yoshiaki
机构信息
Department of Ophthalmology, Hiroshima Memorial Hospital, 1-4-3, Honkawacho, Naka-ku, Hiroshima 730-0802, Japan.
Department of Ophthalmology, Graduate School of Medical Sciences, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
出版信息
J Ophthalmol. 2019 May 21;2019:2431481. doi: 10.1155/2019/2431481. eCollection 2019.
PURPOSE
To investigate the effect of ocular hypertension-induced isomerization of aspartic acid in retinal proteins.
METHODS
Adult Wistar rats with ocular hypertension were used as an experimental model. D--aspartic acid-containing proteins were isolated by SDS-PAGE and western blot with an anti-D--aspartic acid antibody and identified by liquid chromatography-mass spectrometry analysis. The concentration of ATP was measured by ELISA.
RESULTS
D--aspartic acid was expressed in a protein band at around 44.5 kDa at much higher quantities in the retinas of rats with ocular hypertension than in those of normotensive rats. The 44.5 kDa protein band was mainly composed of -enolase, S-arrestin, and ATP synthase subunits and , in both the ocular hypertensive and normotensive retinas. Moreover, increasing intraocular pressure was correlated with increasing ATP concentrations in the retinas of rats.
CONCLUSION
Ocular hypertension affected the expression of proteins containing D--aspartic acid, including ATP synthase subunits, and up-regulation of ATP in the retinas of rats.
目的
研究高眼压诱导视网膜蛋白中天冬氨酸异构化的作用。
方法
将成年高眼压Wistar大鼠作为实验模型。通过SDS-PAGE和用抗D-天冬氨酸抗体进行的蛋白质印迹法分离含D-天冬氨酸的蛋白质，并通过液相色谱-质谱分析进行鉴定。通过ELISA测定ATP浓度。
结果
在高眼压大鼠视网膜中，D-天冬氨酸在约44.5 kDa的蛋白条带中表达，其含量远高于正常血压大鼠视网膜中的含量。在高眼压和正常血压视网膜中，44.5 kDa蛋白条带主要由烯醇化酶、S- arrestin和ATP合酶亚基α和β组成。此外，大鼠视网膜中眼压升高与ATP浓度增加相关。
结论
高眼压影响含D-天冬氨酸蛋白质的表达，包括ATP合酶亚基，并使大鼠视网膜中ATP上调。
Zotero 插件