Department of Oral Pathology, School of Dentistry, Universidade Federal do Rio Grande do Sul, Porto Alegre.
Departments of Stomatology.
Appl Immunohistochem Mol Morphol. 2020 Aug;28(7):513-517. doi: 10.1097/PAI.0000000000000785.
Podoplanin is a transmembrane glycoprotein expressed on various normal or neoplastic cells. Some studies have shown that podoplanin promotes the migration and invasion of tumor cells. This study evaluated a podoplanin expression in Odontogenic Keratocysts (OKs) associated or not associated with Nevoid Basal Cell Carcinoma Syndrome (NBCCS) and in Orthokeratinized Odontogenic Cysts (OOCs).
A total of 50 lesions were obtained in this study, 28 OKs, 18 OKs associated with NBCCS, and 4 OOCs. Immunohistochemical expression of podoplanin in epithelial cells was evaluated using the following score: (a) intensity of immunostaining: (0: absent, 1: weak, 2: moderate, 3: strong, and 4: very strong) and (b) number of positively cells (0: 0%, 1: <25%, 2: 25% to 50%, 3: 50% to 75%, and 4: >75%). The final score was determined by adding the scores of a and b and ranged from 0 to 8 (0: absent, 1 to 4: weak, and 5 to 8: strong).
Podoplanin expression was significantly stronger in the basal layer OKs and NBCCS lesions. Further, podoplanin expression was the highest in the suprabasal layer of NBCCS lesions, followed by the suprabasal layers of OK and OOC lesions.
Podoplanin expression is different in lesions of different biological behaviors. Podoplanin seems to play a role in cell proliferation and migration.
Podoplanin 是一种表达于各种正常或肿瘤细胞的跨膜糖蛋白。一些研究表明 Podoplanin 促进肿瘤细胞的迁移和侵袭。本研究评估了牙源性角化囊肿（OKC）中是否存在与神经嵴基底细胞痣综合征（NBCCS）相关的 Podoplanin 表达，以及与 OKC 相关的 Orthokeratinized Odontogenic Cysts（OOCs）。
本研究共获得 50 例病变，其中 28 例 OKC、18 例与 NBCCS 相关的 OKC 和 4 例 OOC。采用以下评分评估上皮细胞中 Podoplanin 的免疫组织化学表达：（a）免疫染色强度：（0：无，1：弱，2：中度，3：强，4：很强）和（b）阳性细胞数（0：0%，1：<25%，2：25%至 50%，3：50%至 75%，4：>75%）。最终评分通过将 a 和 b 的评分相加确定，范围为 0 至 8（0：无，1 至 4：弱，5 至 8：强）。
Podoplanin 在 OKC 和 NBCCS 病变的基底细胞层中表达明显更强。此外，NBCCS 病变的超基底细胞层中 Podoplanin 表达最高，其次是 OK 和 OOC 病变的超基底细胞层。
Podoplanin 的表达在不同生物学行为的病变中不同。Podoplanin 似乎在细胞增殖和迁移中发挥作用。
