Department of Stomatology, Area of Pathology, Bauru School of Dentistry, University of São Paulo, Bauru, São Paulo, Brazil.
Arch Oral Biol. 2013 Apr;58(4):408-15. doi: 10.1016/j.archoralbio.2012.06.002. Epub 2012 Jul 3.
To investigate podoplanin expression in epithelial odontogenic tumours with and without ectomesenchyme and verify the association between its immunoexpression and proliferative activity in keratocystic odontogenic tumours (KCOTS) and orthokeratinized odontogenic cysts (OOCs).
Eight ameloblastomas, nine adenomatoid odontogenic tumours, twenty KCOTS, five OOC, one calcifying epithelial odontogenic tumour, two ameloblastic fibromas, four ameloblastic fibro-odontomas and five calcifying cystic odontogenic tumours were immunohistochemically analysed with anti-podoplanin antibody. For KCOTS and OOC, the cell proliferation index was determined with Ki-67 immunostaining and compared by Spearman correlation coefficient.
Podoplanin was expressed in the peripheral odontogenic epithelium of most tumours. Ectomesenchyme was negative, except for odontoblasts. KCOTS exhibited positive podoplanin expression while in OOC it was absent/weak. There was statistically significant correlation (p=0.006) between podoplanin expression and cellular proliferation index of KCOTS and OOC.
Podoplanin seems to be related to the proliferative activity of KCOTS and may have a role in the process of local invasion of odontogenic tumours with and without ectomesenchyme.
研究伴有和不伴有中胚层的上皮性牙源性肿瘤中波形蛋白的表达，并验证其在牙源性角化囊性瘤（KCOTs）和正角化牙源性囊肿（OOCs）中的免疫表达与增殖活性之间的相关性。
对 8 例造釉细胞瘤、9 例腺牙源性肿瘤、20 例 KCOTs、5 例 OOC、1 例钙化上皮性牙源性肿瘤、2 例成釉细胞瘤纤维瘤、4 例成釉细胞瘤纤维-牙瘤和 5 例牙源性钙化囊性瘤进行抗波形蛋白抗体免疫组织化学分析。对于 KCOTs 和 OOC，用 Ki-67 免疫染色法测定细胞增殖指数，并通过 Spearman 相关系数进行比较。
波形蛋白在大多数肿瘤的周围牙源性上皮中表达。中胚层除成牙本质细胞外均为阴性。KCOTs 表现出阳性的波形蛋白表达，而在 OOC 中则为阴性/弱阳性。KCOTs 和 OOC 的波形蛋白表达与细胞增殖指数之间存在统计学显著相关性（p=0.006）。
波形蛋白似乎与 KCOTs 的增殖活性有关，可能在伴有和不伴有中胚层的牙源性肿瘤的局部侵袭过程中起作用。
