Yunnan Agricultural University , Kunming 650201 , China.
Microorganism Fermentation Engineer Research Center of Yunnan , Kunming 650217 , China.
J Agric Food Chem. 2018 Oct 3;66(39):10163-10168. doi: 10.1021/acs.jafc.8b03806. Epub 2018 Sep 19.
Bioassay-guided fractionation of the supernatant of the biocontrol strain Bacillus amyloliquefaciens W1 led to the isolation of eight acaricidal cyclodipeptides from the active fractions by column chromatography separation and HPLC purification. The chemical structures of these compounds were identified as cyclo-(Gly-l-Phe), 2, cyclo-(l-Phe- trans-4-OH-l-Pro), 3, cyclo-(Gly-l-Tyr), 4, cyclo-(l-Ala-l-Pro), 5, cyclo-(l-Pro- trans-4-OH-l-Pro), 6, cyclo-(Gly-l-Pro), 7, cyclo-(l-Pro-l-Pro), 8, and cyclo-(l-Tyr- trans-4-OH-l-Pro), 9. Those cyclodipeptides displayed significant acaricidal activities with LC values of 13.85-98.24 μM. Cyclo-(l-Tyr- trans-4-OH-l-Pro) (LC 13.85 μM) was five times more effective than the positive control abamectin (LC 72.06 μM). The results indicated that the hydroxyl group is an important component. This is the first report on the acaricidal capabilities of cyclodipeptides against Tetranychus urticae. The results revealed that the acaricidal activity of the biocontrol strain B. amyloliquefaciens W1 was dependent on its constituent cyclodipeptides, which have the potential to be safe and environmentally friendly acaricides.
生物测定指导下的生防菌株解淀粉芽孢杆菌 W1 上清液的分段分离，通过柱层析分离和 HPLC 纯化，从活性部位分离出 8 种杀螨环二肽。这些化合物的化学结构被鉴定为环-(甘-苯丙氨酸)，2，环-(苯丙氨酸-反式-4-羟基-脯氨酸)，3，环-(甘-酪氨酸)，4，环-(丙氨酸-脯氨酸)，5，环-(脯氨酸-反式-4-羟基-脯氨酸)，6，环-(甘-脯氨酸)，7，环-(脯氨酸-脯氨酸)，8，和环-(酪氨酸-反式-4-羟基-脯氨酸)，9。这些环二肽表现出显著的杀螨活性，LC 值为 13.85-98.24 μM。环-(酪氨酸-反式-4-羟基-脯氨酸)（LC 值为 13.85 μM）比阳性对照阿维菌素（LC 值为 72.06 μM）有效 5 倍。结果表明，羟基是一个重要的组成部分。这是环二肽对二斑叶螨具有杀螨活性的首次报道。结果表明，生防菌株解淀粉芽孢杆菌 W1 的杀螨活性依赖于其组成的环二肽，这些环二肽具有成为安全、环保杀螨剂的潜力。
