Casanellas Ignasi, Lagunas Anna, Vida Yolanda, Pérez-Inestrosa Ezequiel, Andrades José A, Becerra José, Samitier Josep
Institute for Bioengineering of Catalonia (IBEC), Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain.
Department of Electronics and Biomedical Engineering, University of Barcelona (UB), 08028 Barcelona, Spain.
Biomimetics (Basel). 2019 Jun 25;4(2):43. doi: 10.3390/biomimetics4020043.
Extracellular matrix remodeling plays a pivotal role during mesenchyme patterning into different lineages. Tension exerted from cell membrane receptors bound to extracellular matrix ligands is transmitted by the cytoskeleton to the cell nucleus inducing gene expression. Here, we used dendrimer-based arginine-glycine-aspartic acid (RGD) uneven nanopatterns, which allow the control of local surface adhesiveness at the nanoscale, to unveil the adhesive requirements of mesenchymal tenogenic and osteogenic commitments. Cell response was found to depend on the tension resulting from cell-substrate interactions, which affects nuclear morphology and is regulated by focal adhesion size and distribution.
细胞外基质重塑在间充质分化为不同谱系的过程中起着关键作用。与细胞外基质配体结合的细胞膜受体所施加的张力通过细胞骨架传递到细胞核，从而诱导基因表达。在此，我们使用基于树枝状聚合物的精氨酸-甘氨酸-天冬氨酸（RGD）不均匀纳米图案，其能够在纳米尺度上控制局部表面粘附性，以揭示间充质向肌腱和骨生成方向分化时的粘附需求。研究发现细胞反应取决于细胞与底物相互作用产生的张力，这种张力会影响细胞核形态，并受粘着斑大小和分布的调节。
