Department of Solid State Engineering, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic.
Department of Biochemistry and Microbiology, University of Chemistry and Technology Prague, 166 28 Prague, Czech Republic.
Int J Mol Sci. 2022 Apr 23;23(9):4676. doi: 10.3390/ijms23094676.
Here, we report on the nanopatterning of different aromatic polymer substrates achieved by KrF excimer laser treatment. The conditions for the construction of the laser-induced periodic surface structures, the so-called LIPSS pattern, were established by optimized laser fluence and a number of pulses. The polymer substrates were polyethylene naphthalate (PEN), polyethersulfone (PES), and polystyrene (PS), which were chosen since they are thermally, chemically, and mechanically resistant polymers with high absorption coefficients at the excimer laser wavelength. The surface morphology of the treated substrates was investigated by atomic force microscopy and scanning electron microscopy, and the roughness and effective surface area on the modified samples were determined. Elemental concentration was characterized by energy-dispersive (EDX) analysis, surface chemistry was determined with X-ray photoelectron spectroscopy (XPS). The samples with the formation of LIPSS induced by 10 mJ·cm with 1000, 3000, and 6000 pulses were used for subsequent in vitro cytocompatibility tests using human cells from osteosarcoma (U-2 OS). The LIPSS pattern and its ability of significant cell guidance were confirmed for some of the studied samples. Cell morphology, adhesion, and proliferation were evaluated. The results strongly contribute to the development of novel applications using nanopatterned polymers, e.g., in tissue engineering, cell analysis or in combination with metallization for sensor construction.
在这里，我们报告了不同芳香族聚合物基底的纳米图案化，这是通过 KrF 准分子激光处理实现的。通过优化激光能量密度和脉冲数，确定了构建激光诱导周期性表面结构（所谓的 LIPSS 图案）的条件。所选聚合物基底为聚萘二甲酸乙二醇酯（PEN）、聚醚砜（PES）和聚苯乙烯（PS），因为它们是热稳定、化学稳定和机械稳定的聚合物，在准分子激光波长下具有高吸收率。通过原子力显微镜和扫描电子显微镜研究了处理后的基底的表面形貌，并确定了改性样品的粗糙度和有效表面积。通过能谱（EDX）分析对元素浓度进行了表征，通过 X 射线光电子能谱（XPS）确定了表面化学性质。用 10 mJ·cm 的激光能量密度和 1000、3000 和 6000 个脉冲诱导形成 LIPSS 的样品用于随后的体外细胞相容性测试，使用骨肉瘤（U-2 OS）的人细胞进行测试。证实了一些研究样品中 LIPSS 图案及其对细胞的显著引导能力。评估了细胞形态、黏附和增殖。这些结果为使用纳米图案聚合物的新型应用（例如组织工程、细胞分析或与金属化结合用于传感器构建）的发展提供了有力支持。
