Kryuchkov Mikhail, Wang Zhehui, Valnohova Jana, Savitsky Vladimir, Karamehmedović Mirza, Jobin Marc, Katanaev Vladimir L
Department of Cell Physiology and Metabolism, Faculty of Medicine, University of Geneva, Rue Michel Servet 1, CH-1211 Geneva, Switzerland.
School of Chemistry and Pharmaceutical Engineering, Shandong First Medical University (Shandong Academy of Medical Sciences), Tai'an 271016, China.
Biomimetics (Basel). 2025 Mar 7;10(3):163. doi: 10.3390/biomimetics10030163.
Nanopatterning of signal-transmitting proteins is essential for cell physiology and drug delivery but faces challenges such as high cost, limited pattern variability, and non-biofriendly materials. Arthropods, particularly beetles (Coleoptera), offer a natural model for biomimetic nanopatterning due to their diverse corneal nanostructures. Using atomic force microscopy (AFM), we analyzed Coleoptera corneal nanocoatings and identified dimpled nanostructures that can transform into maze-like/nipple-like protrusions. Further analysis suggested that these modifications result from a temporary, self-assembled process influenced by surface adhesion. We identified cuticular protein 7 (CP7) as a key component of dimpled nanocoatings. Biophysical analysis revealed CP7's unique self-assembly properties, allowing us to replicate its nanopatterning ability in vitro. Our findings demonstrate CP7's potential for bioinspired nanocoatings and provide insights into the evolutionary mechanisms of nanostructure formation. This research paves the way for cost-effective, biomimetic nanopatterning strategies with applications in nanotechnology and biomedicine.
信号传递蛋白的纳米图案化对细胞生理学和药物递送至关重要，但面临着成本高、图案可变性有限和材料不具生物友好性等挑战。节肢动物，特别是甲虫（鞘翅目），由于其多样的角膜纳米结构，为仿生纳米图案化提供了一个天然模型。我们使用原子力显微镜（AFM）分析了鞘翅目昆虫的角膜纳米涂层，并识别出了可转变为迷宫状/乳头状突起的凹坑状纳米结构。进一步分析表明，这些修饰是由受表面粘附影响的临时自组装过程导致的。我们确定表皮蛋白7（CP7）是凹坑状纳米涂层的关键成分。生物物理分析揭示了CP7独特的自组装特性，使我们能够在体外复制其纳米图案化能力。我们的研究结果证明了CP7在生物启发纳米涂层方面的潜力，并为纳米结构形成的进化机制提供了见解。这项研究为具有成本效益的仿生纳米图案化策略铺平了道路，这些策略可应用于纳米技术和生物医学领域。
