Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Genes (Basel). 2019 Jun 25;10(6):481. doi: 10.3390/genes10060481.
The damage caused by is the main factor threatening the production of vegetables in the Liliaceae family. However, few genetic studies of have been conducted because of a lack of genomic resources. Many long-read sequencing technologies have been developed in the last decade; therefore, in this study, the transcriptome including all development stages of was sequenced for the first time by Pacific single-molecule long-read sequencing. Here, 39,129 isoforms were generated, and 35,645 were found to have annotation results when checked against sequences available in different databases. Overall, 18,473 isoforms were distributed in 25 various Clusters of Orthologous Groups, and 11,880 isoforms were categorized into 60 functional groups that belonged to the three main Gene Ontology classifications. Moreover, 30,610 isoforms were assigned into 44 functional categories belonging to six main Kyoto Encyclopedia of Genes and Genomes functional categories. Coding DNA sequence (CDS) prediction showed that 36,419 out of 39,129 isoforms were predicted to have CDS, and 4319 simple sequence repeats were detected in total. Finally, 266 insecticide resistance and metabolism-related isoforms were identified as candidate genes for further investigation of insecticide resistance and metabolism in .
是造成百合科蔬菜减产的主要威胁因素，但由于基因组资源匮乏，针对的遗传研究较少。在过去十年中，已经开发出许多长读长测序技术；因此，在本研究中，首次通过太平洋单分子长读长测序对包括所有发育阶段的转录组进行了测序。在这里，生成了 39129 个异构体，当与不同数据库中的序列进行比对时，发现 35645 个具有注释结果。总体而言，18473 个异构体分布在 25 个不同的同源簇中，11880 个异构体分为 60 个功能组，属于三个主要基因本体论分类。此外，30610 个异构体被分配到 44 个功能类别，属于六个主要京都基因与基因组百科全书功能类别。编码 DNA 序列 (CDS) 预测显示，在 39129 个异构体中，有 36419 个被预测具有 CDS，总共检测到 4319 个简单重复序列。最后，鉴定出 266 个与杀虫剂抗性和代谢相关的异构体，作为进一步研究在 中杀虫剂抗性和代谢的候选基因。
