Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230031, China.
Department of Agriculture, Abdul Wali Khan University, Mardan 23200, Khyber Pakhtunkhwa, Pakistan.
Int J Mol Sci. 2017 Nov 17;18(11):2445. doi: 10.3390/ijms18112445.
(Diptera: Sciaridae) is the most important pest of Chinese chive. Insecticides are used widely and frequently to control . in China. However, the performance of the insecticides chlorpyrifos and clothianidin in controlling the Chinese chive maggot is quite different. Using next generation sequencing technology, different expression unigenes (DEUs) in were detected after treatment with chlorpyrifos and clothianidin for 6 and 48 h in comparison with control. The number of DEUs ranged between 703 and 1161 after insecticide treatment. In these DEUs, 370-863 unigenes can be classified into 41-46 categories of gene ontology (GO), and 354-658 DEUs can be mapped into 987-1623 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expressions of DEUs related to insecticide-metabolism-related genes were analyzed. The cytochrome P450-like unigene group was the largest group in DEUs. Most glutathione S-transferase-like unigenes were down-regulated and most sodium channel-like unigenes were up-regulated after insecticide treatment. Finally, 14 insecticide-metabolism-related unigenes were chosen to confirm the relative expression in each treatment by quantitative Real Time Polymerase Chain Reaction (qRT-PCR). The results of qRT-PCR and RNA Sequencing (RNA-Seq) are fairly well-established. Our results demonstrate that a next-generation sequencing tool facilitates the identification of insecticide-metabolism-related genes and the illustration of the insecticide mechanisms of chlorpyrifos and clothianidin.
（双翅目：菌蚊科）是中国韭菜最重要的害虫。在中国，广泛而频繁地使用杀虫剂来控制。然而，毒死蜱和噻虫嗪在防治韭菜蛆方面的表现却大不相同。采用下一代测序技术，在与对照相比，用毒死蜱和噻虫嗪处理 6 和 48 小时后，检测到不同表达的 unigenes（DEUs）。在这些 DEUs 中，370-863 个 unigenes 可以分为 41-46 个基因本体论（GO）类别，354-658 个 DEUs 可以映射到 987-1623 个京都基因与基因组百科全书（KEGG）途径。分析了与杀虫剂代谢相关基因的 DEUs 表达情况。细胞色素 P450 样 unigene 组是 DEUs 中最大的一组。大多数谷胱甘肽 S-转移酶样 unigenes下调，大多数钠通道样 unigenes上调后杀虫剂处理。最后，选择了 14 个杀虫剂代谢相关 unigenes，通过定量实时聚合酶链反应（qRT-PCR）在每个处理中确认相对表达量。qRT-PCR 和 RNA 测序（RNA-Seq）的结果相当一致。我们的结果表明，下一代测序工具有助于鉴定杀虫剂代谢相关基因，并阐明毒死蜱和噻虫嗪的杀虫剂机制。
