Hammam Nevin, Salah Shaimaa, Kholef Emad F, Moussa Ehab M, Marotta Anthony
Department of Rheumatology and Rehabilitation, Assiut University Hospitals, Assiut, Egypt.
Faculty of Medicine, University of Alberta, Edmonton, AB, Canada.
Mod Rheumatol. 2020 Jul;30(4):664-670. doi: 10.1080/14397595.2019.1637575. Epub 2019 Jul 22.
The aim of the study is to examine the association between 14-3-3η protein levels in both serum and synovial fluid (SF) with various parameters in a longitudinal cohort of patients with established rheumatoid arthritis (RA). Serum and SF samples were obtained from RA patients and 14-3-3η levels were measured. Radiological damage and progression were evaluated using Sharp/van der Heijde score (SHS) at study entry and at 2-years follow-up. A total of 39 RA patients were included with a mean disease duration of 9.6 ± 8 years. Levels of 14-3-3η were two-fold higher in SF than in serum (mean of 3.7 versus 1.7 ng/mL, respectively). While no significant association was found between 14-3-3η levels with disease activity or other laboratory assessments, both serum and SF 14-3-3η levels positively correlated with radiographic damage at baseline (SHS; < .001). SF, but not serum, 14-3-3η levels correlated with absolute progression ( < .03). 14-3-3η levels are significantly higher in RA SF than in serum in an established RA cohort. Serum and SF 14-3-3η levels correlate with radiographic damage at baseline and at 2-years follow-up. This study further substantiates the utility of 14-3-3η as a biomarker for mechanistic joint damage in established RA.
本研究的目的是在一个患有确诊类风湿性关节炎（RA）的纵向队列患者中，检测血清和滑液（SF）中14-3-3η蛋白水平与各种参数之间的关联。从RA患者中获取血清和SF样本，并测量14-3-3η水平。在研究开始时和2年随访时，使用Sharp/van der Heijde评分（SHS）评估放射学损伤和进展情况。共纳入39例RA患者，平均病程为9.6±8年。SF中14-3-3η水平比血清中高两倍（分别为3.7 ng/mL和1.7 ng/mL）。虽然未发现14-3-3η水平与疾病活动度或其他实验室评估之间存在显著关联，但血清和SF中的14-3-3η水平在基线时均与放射学损伤呈正相关（SHS；<0.001）。SF中的14-3-3η水平与绝对进展相关（<0.03），而血清中的则不然。在一个确诊RA队列中，RA患者SF中的14-3-3η水平显著高于血清中的。血清和SF中的~14-3-3η水平在基线时和2年随访时均与放射学损伤相关。本研究进一步证实了14-3-3η作为确诊RA中机械性关节损伤生物标志物的实用性。
