College of Life Science, Northwest Normal University, 967 Anning East Road, Anning District, Lanzhou, 730070, People's Republic of China.
Bioactive Products Engineering Research Center For Gansu Distinctive Plants, Northwest Normal University, Lanzhou, 730070, People's Republic of China.
Curr Microbiol. 2019 Sep;76(9):1066-1072. doi: 10.1007/s00284-019-01721-9. Epub 2019 Jun 26.
Seven endophytic yeast strains were isolated from tangerine peel (Citrus reticulata Blanco) and genotyped through clustering with D1/D2 and ITS1-5.8S-ITS2 sequences from GenBank. Phenotypic characteristics were obtained through commercial kits and through assisted species identification. Indole-3-acetic acid (IAA) production by the yeast strains was assessed using Salkowski reagent and High-Performance Liquid chromatography (HPLC). The growth-promoting effects of the yeast were evaluated using the 'ragdoll' method. CRYb1, CRYb2 and CRYb7 isolates were identified as the closest species Hanseniaspora opuntiae. CRYb3 was identified as Pichia kluyveri. CRYb4, CRYb5 and CRYb6 were identified as Meyerozyma guilliermondii. CRYb1, CRYb5, CRYb6 and CRYb7 were found to be capable of IAA production. The most promising yeast strains now require further evaluation for their ability to promote plant growth in vitro and in vivo. These data increase our knowledge of the distribution and biological properties of endophytic yeast. This is important information that will be required to fully harness the growth-promoting properties of yeast strains.
从陈皮（Citrus reticulata Blanco）中分离出 7 株内生酵母菌株，并通过聚类分析 GenBank 中的 D1/D2 和 ITS1-5.8S-ITS2 序列进行基因分型。通过商业试剂盒和辅助物种鉴定获得表型特征。使用 Salkowski 试剂和高效液相色谱法（HPLC）评估酵母菌株的吲哚-3-乙酸（IAA）产量。使用“布娃娃”方法评估酵母的促生长作用。CRYb1、CRYb2 和 CRYb7 分离株被鉴定为最接近的物种汉逊酵母（Hanseniaspora opuntiae）。CRYb3 被鉴定为毕赤酵母（Pichia kluyveri）。CRYb4、CRYb5 和 CRYb6 被鉴定为假丝酵母（Meyerozyma guilliermondii）。CRYb1、CRYb5、CRYb6 和 CRYb7 被发现能够产生 IAA。目前最有前途的酵母菌株需要进一步评估其在体外和体内促进植物生长的能力。这些数据增加了我们对内生酵母分布和生物学特性的了解。这是充分利用酵母菌株促生长特性所需的重要信息。
