Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Hebei Key Laboratory of Research and Development for Traditional Chinese Medicine, Chengde Medical University, Chengde, Hebei, P. R. China.
Phytochem Anal. 2020 Jan;31(1):15-27. doi: 10.1002/pca.2861. Epub 2019 Jun 26.
Scurrula ferruginea (Jack) Danser is a hemi-parasitic shrub that is widely used as a traditional herbal medicine.
To elucidate the anti-inflammatory activity of S. ferruginea parasitising on three different hosts of Vitex negundo L., Micromelum minutum (G. Forst.) Wight & Arn. and Tecoma stans (L.) Juss ex HBK., as well as, to determine the metabolite differences related to their anti-inflammatory properties.
Two plant parts of S. ferruginea, stems and leaves, were extracted in water. The freeze-dried stem of S. ferruginea grown on T. stans was liquid-liquid partitioned into several solvents. Their potential anti-inflammatory activity was assessed via inhibition of nitric oxide (NO) production in lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-induced RAW 264.7 macrophage cells. The metabolite variation was examined using proton nuclear magnetic resonance ( H-NMR) combined with multivariate data analysis (MVDA).
Scurrula ferruginea stems parasitising on T. stans and V. negundo which were freeze dried exhibited higher anti-inflammatory activity with IC values of 114.47 ± 2.96 and 118.87 ± 2.31 μg/mL, respectively. The mid-polar ethyl acetate fraction of S. ferruginea hosted on T. stans displayed the highest NO inhibition with 84.80 ± 0.45% at 200 μg/mL. Principal component analysis (PCA) indicated notable and clear discriminations among the different plant parts and host plants based on the identified metabolites. Furthermore, partial least squares (PLS) regression model suggested the anti-inflammatory bioactivity might be associated with the presence of choline, isoleucine, catechin, leucine and chlorogenic acid.
This study suggests S. ferruginea could serve as a potential anti-inflammatory agent, highlighting the importance of T. stans as the host plant.
Scurrula ferruginea（Jack）Danser 是一种半寄生灌木，广泛用作传统草药。
阐明寄生在 Vitex negundo L.、Micromelum minutum（G. Forst.）Wight & Arn. 和 Tecoma stans（L.）Juss ex HBK. 三种不同宿主上的 S. ferruginea 的抗炎活性，并确定与其抗炎特性相关的代谢物差异。
S. ferruginea 的两个植物部分，茎和叶，用水提取。寄生在 T. stans 上的 S. ferruginea 干燥茎进行液-液分配，分成几个溶剂。通过抑制脂多糖（LPS）和干扰素-γ（IFN-γ）诱导的 RAW 264.7 巨噬细胞中一氧化氮（NO）的产生来评估它们的潜在抗炎活性。使用质子核磁共振（H-NMR）结合多变量数据分析（MVDA）检查代谢物的变化。
寄生在 T. stans 和 V. negundo 上的 S. ferruginea 干燥茎表现出更高的抗炎活性，IC 值分别为 114.47±2.96μg/mL 和 118.87±2.31μg/mL。寄生在 T. stans 上的 S. ferruginea 的中极性乙酸乙酯部分在 200μg/mL 时表现出最高的 NO 抑制作用，为 84.80±0.45%。主成分分析（PCA）表明，基于鉴定的代谢物，不同的植物部分和宿主植物之间存在明显而清晰的区分。此外，偏最小二乘（PLS）回归模型表明，抗炎生物活性可能与胆碱、异亮氨酸、儿茶素、亮氨酸和绿原酸的存在有关。
本研究表明 S. ferruginea 可以作为一种潜在的抗炎剂，强调了 T. stans 作为宿主植物的重要性。
