Mediani Ahmed, Abas Faridah, Maulidiani M, Khatib Alfi, Tan Chin Ping, Ismail Intan Safinar, Shaari Khozirah, Ismail Amin
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
Molecules. 2017 May 30;22(6):902. doi: 10.3390/molecules22060902.
is an important medicinal plant. To standardize the extract and guarantee its maximum benefit, processing methods optimization ought to be amenable and beneficial. Herein, three dried samples, air (AD), freeze (FD) and oven (OD), extracted with various ethanol to water ratios (0%, 50%, 70%, 80% and 100%) were evaluated for their metabolite changes using proton nuclear magnetic resonance (¹H-NMR)-based metabolomics approach. The amino acids analysis showed that FD exhibited higher content of most amino acids compared to the other dried samples. Based on principal component analysis (PCA), the FD extracted with 80% ethanol contained higher amounts of hypophyllanthin and phenolic compounds based on the loading plot. The partial least-square (PLS) results showed that the phytochemicals, including hypophyllanthin, catechin, epicatechin, rutin, quercetin and chlorogenic, caffeic, malic and gallic acids were correlated with antioxidant and α-glucosidase inhibitory activities, which were higher in the FD material extracted with 80% ethanol. This report optimized the effect of drying and ethanol ratios and these findings demonstrate that NMR-based metabolomics was an applicable approach. The FD extracted with 80% ethanol can be used as afunctional food ingredient for nutraceutical or in medicinal preparation.
是一种重要的药用植物。为了规范提取物并确保其最大益处，加工方法的优化应该是可行且有益的。在此，使用基于质子核磁共振（¹H-NMR）的代谢组学方法，对三个干燥样品，即风干（AD）、冻干（FD）和烘干（OD）样品，用不同乙醇与水比例（0%、50%、70%、80%和100%）进行提取，以评估其代谢物变化。氨基酸分析表明，与其他干燥样品相比，FD样品中大多数氨基酸的含量更高。基于主成分分析（PCA），从载荷图来看，用80%乙醇提取的FD样品含有较高含量的叶下珠素和酚类化合物。偏最小二乘法（PLS）结果表明，包括叶下珠素、儿茶素、表儿茶素、芦丁、槲皮素以及绿原酸、咖啡酸、苹果酸和没食子酸在内的植物化学物质与抗氧化和α-葡萄糖苷酶抑制活性相关，在用80%乙醇提取的FD材料中这些活性更高。本报告优化了干燥和乙醇比例的影响，这些发现表明基于核磁共振的代谢组学是一种适用的方法。用80%乙醇提取的FD样品可作为营养保健品的功能性食品成分或用于药物制剂。
