School of Engineering and Materials Science , Queen Mary University of London , London , E1 4NS , United Kingdom.
Centre of Foundation Studies , Universiti Teknologi MARA , Cawangan Selangor, Kampus Dengkil , 43800 Dengkil , Selangor , Malaysia.
Biomacromolecules. 2019 Jul 8;20(7):2506-2514. doi: 10.1021/acs.biomac.9b00245. Epub 2019 Jun 20.
Inflammatory conditions are frequently accompanied by increased levels of active proteases, and there is rising interest in methods for their detection to monitor inflammation in a point of care setting. In this work, new sensor materials for disposable single-step protease biosensors based on poly(2-oxazoline) hydrogels cross-linked with a protease-specific cleavable peptide are described. The performance of the sensor material was assessed targeting the detection of matrix metalloproteinase-9 (MMP-9), a protease that has been shown to be an indicator of inflammation in multiple sclerosis and other inflammatory conditions. Films of the hydrogel were formed on gold-coated quartz crystals using thiol-ene click chemistry, and the cross-link density was optimized. The degradation rate of the hydrogel was monitored using a quartz crystal microbalance (QCM) and showed a strong dependence on the MMP-9 concentration. A concentration range of 0-160 nM of MMP-9 was investigated, and a lower limit of detection of 10 nM MMP-9 was determined.
炎症状态通常伴随着活性蛋白酶水平的升高，因此人们越来越感兴趣于开发用于检测的方法，以便在即时护理环境中监测炎症。在这项工作中，描述了一种新的传感器材料，用于基于聚(2-恶唑啉)水凝胶与蛋白酶特异性可切割肽交联的一次性单步蛋白酶生物传感器。该传感器材料的性能通过检测基质金属蛋白酶-9（MMP-9）来评估，MMP-9 已被证明是多发性硬化症和其他炎症状态的炎症标志物。使用硫醇-烯点击化学在镀金石英晶体上形成水凝胶膜，并对交联密度进行了优化。水凝胶的降解速率通过石英晶体微天平（QCM）进行监测，结果表明其强烈依赖于 MMP-9 的浓度。研究了 MMP-9 的浓度范围为 0-160 nM，确定了 MMP-9 的检测下限为 10 nM。
