Department of Orthopaedics, Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, 197 Ruijin 2nd Road, 200025, Shanghai, P. R. China.
Department of Orthopaedics, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, 85 Wujin Road, 200080, Shanghai, P. R. China.
Nat Commun. 2022 Jan 10;13(1):160. doi: 10.1038/s41467-021-27816-1.
Immune response and new tissue formation are important aspects of tissue repair. However, only a single aspect is generally considered in previous biomedical interventions, and the synergistic effect is unclear. Here, a dual-effect coating with immobilized immunomodulatory metal ions (e.g., Zn) and osteoinductive growth factors (e.g., BMP-2 peptide) is designed via mussel adhesion-mediated ion coordination and molecular clicking strategy. Compared to the bare TiO group, Zn can increase M2 macrophage recruitment by up to 92.5% in vivo and upregulate the expression of M2 cytokine IL-10 by 84.5%; while the dual-effect of Zn and BMP-2 peptide can increase M2 macrophages recruitment by up to 124.7% in vivo and upregulate the expression of M2 cytokine IL-10 by 171%. These benefits eventually significantly enhance bone-implant mechanical fixation (203.3 N) and new bone ingrowth (82.1%) compared to the bare TiO (98.6 N and 45.1%, respectively). Taken together, the dual-effect coating can be utilized to synergistically modulate the osteoimmune microenvironment at the bone-implant interface, enhancing bone regeneration for successful implantation.
免疫反应和新组织形成是组织修复的重要方面。然而，以前的生物医学干预通常只考虑到其中一个方面，协同作用尚不清楚。在这里，通过贻贝黏附介导的离子配位和分子点击策略设计了一种具有固定化免疫调节金属离子（例如 Zn）和骨诱导生长因子（例如 BMP-2 肽）的双效涂层。与裸 TiO 组相比，Zn 可使体内 M2 巨噬细胞募集增加高达 92.5%，并使 M2 细胞因子 IL-10 的表达上调 84.5%；而 Zn 和 BMP-2 肽的双重作用可使体内 M2 巨噬细胞募集增加高达 124.7%，并使 M2 细胞因子 IL-10 的表达上调 171%。这些益处最终可显著提高骨-植入物的机械固定（203.3 N）和新骨长入（82.1%），与裸 TiO（分别为 98.6 N 和 45.1%）相比。综上所述，双效涂层可协同调节骨-植入界面的骨免疫微环境，促进骨再生以实现成功植入。
