Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology , Dalian 116024, Liaoning, P.R. China.
Environ Sci Technol. 2017 Sep 19;51(18):10850-10860. doi: 10.1021/acs.est.7b02875. Epub 2017 Aug 30.
Antibiotic resistance genes (ARGs) are globally prevalent in mariculture sediment, and their presence is an issue of concern in the context of antibiotic use. Although large amounts of fishmeal have been released into the sediment, the role of fishmeal in ARG dissemination remains unclear. In this study, high-throughput ARG profiles in representative fishmeal products and the impact of fishmeal on the sediment resistome were investigated. A total of 132 unique ARGs and 4 mobile genetic elements (MGEs) were detected in five fishmeal products. ARG abundance and diversity in the mariculture microcosm sediment were significantly increased by the addition of fishmeal, and trends in ARG patterns correlated with the resident bacterial community in sediment (P < 0.05). After DNase treatment of fishmeal removed 84.3% of total ARGs, the remaining nutrients in fishmeal increased the relative abundance but not the diversity of ARGs in microcosm sediment. Our study has revealed for the first time that fishmeal itself is a major reservoir for ARGs, and the shift in the bacterial community induced by the nutrients in fishmeal is the main driver shaping the resistome in mariculture microcosm sediment. Our findings caution against the previously unperceived risk of ARG propagation in fishmeal-receiving ecosystems.
抗生素耐药基因（ARGs）在海水养殖沉积物中广泛存在，在抗生素使用的背景下，其存在是一个令人关注的问题。尽管已经有大量的鱼粉被释放到沉积物中，但鱼粉在 ARG 传播中的作用仍不清楚。在本研究中，我们调查了具有代表性的鱼粉产品中的高通量 ARG 图谱以及鱼粉对沉积物抗性组的影响。在五种鱼粉产品中检测到了 132 个独特的 ARG 和 4 个移动遗传元件（MGEs）。鱼粉的添加显著增加了养殖微宇宙沉积物中 ARG 的丰度和多样性，ARG 模式的趋势与沉积物中定居细菌群落相关（P < 0.05）。在鱼粉的 DNase 处理去除了 84.3%的总 ARGs 后，鱼粉中剩余的营养物质增加了微宇宙沉积物中 ARG 的相对丰度，但没有增加其多样性。我们的研究首次揭示了鱼粉本身就是 ARGs 的主要储存库，而鱼粉中营养物质引起的细菌群落的变化是塑造海水养殖微宇宙沉积物抗性组的主要驱动力。我们的研究结果提醒人们注意到，在接收鱼粉的生态系统中，ARGs 传播的风险此前尚未被认识到。
