Li Chao, Chen Guoxiong, Mishina Kohei, Yamaji Naoki, Ma Jian Feng, Yukuhiro Fumiko, Tagiri Akemi, Liu Cheng, Pourkheirandish Mohammad, Anwar Nadia, Ohta Masaru, Zhao Pengshan, Lundqvist Udda, Li Xinrong, Komatsuda Takao
National Institute of Agrobiological Sciences Tsukuba Ibaraki Japan.
Shanghai Key Laboratory of Plant Functional Genomics and Resources Shanghai Chenshan Botanical Garden Shanghai China.
Plant Direct. 2017 Nov 3;1(5):e00025. doi: 10.1002/pld3.25. eCollection 2017 Nov.
The hydrophobic cuticle covers the surface of the most aerial organs of land plants. The barley mutant (), which is hypersensitive to drought, is unable to accumulate a sufficient quantity of cutin in its leaf cuticle. The mutated locus has been mapped to a 0.02 cM segment in the pericentromeric region of chromosome 4H. As a map-based cloning approach to isolate the gene was therefore considered unlikely to be feasible, a comparison was instead made between the transcriptomes of the mutant and the wild type. In conjunction with extant genomic information, on the basis of predicted functionality, only two genes were considered likely to encode a product associated with cutin formation. When eight independent mutant alleles were resequenced with respect to the two candidate genes, it was confirmed that the gene underlying the mutation in each allele encodes a Gly-Asp-Ser-Leu (GDSL)-motif esterase/acyltransferase/lipase. The gene was transcribed in the epidermis, and its product was exclusively deposited in cell wall at the boundary of the cuticle in the leaf elongation zone, coinciding with the major site of cutin deposition. CER-ZV is speculated to function in the deposition of cutin polymer. Its homologs were found in green algae, moss, and euphyllophytes, indicating that it is highly conserved in plant kingdom.
疏水角质层覆盖着陆生植物大多数地上器官的表面。大麦突变体（）对干旱高度敏感，其叶片角质层无法积累足够数量的角质。突变位点已被定位到4H染色体着丝粒周围区域的一个0.02 cM片段上。因此，通过图位克隆方法分离该基因被认为不太可行，转而对突变体和野生型的转录组进行了比较。结合现有的基因组信息，基于预测的功能，只有两个基因被认为可能编码与角质形成相关的产物。当对八个独立的突变等位基因就这两个候选基因进行重测序时，证实每个等位基因突变的潜在基因编码一种甘氨酸-天冬氨酸-丝氨酸-亮氨酸（GDSL）基序酯酶/酰基转移酶/脂肪酶。该基因在表皮中表达，其产物仅沉积在叶片伸长区角质层边界的细胞壁中，与角质沉积的主要部位一致。推测CER-ZV在角质聚合物的沉积中起作用。在绿藻、苔藓和真叶植物中发现了它的同源物，表明它在植物界高度保守。
