DiGennaro Peter, Grienenberger Etienne, Dao Thai Q, Jun Ji Hyung, Fletcher Jennifer C
Plant Gene Expression Center USDA-ARS/UC Berkeley Albany California.
Department of Plant and Microbial Biology University of California Berkeley California.
Plant Direct. 2018 Dec 20;2(12):e00103. doi: 10.1002/pld3.103. eCollection 2018 Dec.
Intercellular signaling mediated by small peptides is critical to coordinate organ formation in animals, but whether extracellular polypeptides play similar roles in plants is unknown. Here we describe a role in Arabidopsis leaf development for two members of the CLAVATA3/ESR-RELATED peptide family, CLE5 and CLE6, which lie adjacent to each other on chromosome 2. Uniquely among the genes, and are expressed specifically at the base of developing leaves and floral organs, adjacent to the boundary with the shoot apical meristem. During vegetative development and transcription is regulated by the leaf patterning transcription factors BLADE-ON-PETIOLE1 (BOP1) and ASYMMETRIC LEAVES2 (AS2), as well as by the WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors WOX1 and PRESSED FLOWER (PRS). Moreover, and transcript levels are differentially regulated in various genetic backgrounds by the phytohormone auxin. Analysis of loss-of-function mutations generated by genome engineering reveals that and independently and together have subtle effects on rosette leaf shape. Our study indicates that the CLE5 and CLE6 peptides function downstream of leaf patterning factors and phytohormones to modulate the final leaf morphology.
由小肽介导的细胞间信号传导对于协调动物器官形成至关重要，但细胞外多肽在植物中是否发挥类似作用尚不清楚。在此，我们描述了CLAVATA3/ESR相关肽家族的两个成员CLE5和CLE6在拟南芥叶片发育中的作用，它们在2号染色体上彼此相邻。在这些基因中，CLE5和CLE6独特地在发育中的叶片基部和花器官中特异性表达，与茎尖分生组织的边界相邻。在营养发育过程中，CLE5和CLE6的转录受叶片模式转录因子叶片柄上的叶片1（BOP1）和不对称叶片2（AS2）以及与WUSCHEL相关的同源框（WOX）转录因子WOX1和压花（PRS）的调控。此外，在各种遗传背景下，植物激素生长素对CLE5和CLE6的转录水平有不同的调控作用。对基因组工程产生的功能丧失突变的分析表明，CLE5和CLE6单独或共同对莲座叶形状有细微影响。我们的研究表明，CLE5和CLE6肽在叶片模式因子和植物激素的下游发挥作用，以调节最终的叶片形态。
