Frolova Nadezhda, Gorbach Daria, Ihling Christian, Bilova Tatiana, Orlova Anastasia, Lukasheva Elena, Fedoseeva Ksenia, Dodueva Irina, Lutova Lyudmila A, Frolov Andrej
Laboratory of Analytical Biochemistry and Biotechnology, K.A. Timiryazev Institute of Plant Physiology Russian Academy of Science, 127276 Moscow, Russia.
Institute of Pharmacy, Martin-Luther University Halle-Wittenberg, 06099 Halle (Saale), Germany.
Biomolecules. 2025 Feb 14;15(2):290. doi: 10.3390/biom15020290.
Infection of higher plants with agrobacteria () represents one of the most comprehensively characterized examples of plant-microbial interactions. Incorporation of the bacterial transfer DNA (T-DNA) in the plant genome results in highly efficient expression of the bacterial auxin, cytokinin and opine biosynthesis genes, as well as the host genes of hormone-mediated signaling. These transcriptional events trigger enhanced proliferation of plant cells and formation of crown gall tumors. Because of this, infection of plant tissues with provides a convenient model to address the dynamics of cell metabolism accompanying plant development. To date, both early and late plant responses to agrobacterial infection are well-characterized at the level of the transcriptome, whereas only little information on the accompanying changes in plant metabolism is available. Therefore, here we employ an integrated proteomics and metabolomics approach to address the metabolic shifts and molecular events accompanying plant responses to inoculation with the culture. Based on the acquired proteomics dataset complemented with the results of the metabolite profiling experiment, we succeeded in characterizing the metabolic shifts associated with agrobacterial infection. The observed dynamics of the seedling proteome and metabolome clearly indicated rearrangement of the energy metabolism on the 10th day after inoculation (d.a.i.). Specifically, redirection of the energy metabolism from the oxidative to the anaerobic pathway was observed. This might be a part of the plant's adaptation response to tumor-induced hypoxic stress, which most likely involved activation of sugar signaling.
用农杆菌感染高等植物是植物 - 微生物相互作用中最具全面特征的例子之一。细菌转移DNA（T-DNA）整合到植物基因组中会导致细菌生长素、细胞分裂素和冠瘿碱生物合成基因以及激素介导信号传导的宿主基因高效表达。这些转录事件会引发植物细胞的增殖增强和冠瘿瘤的形成。因此，用农杆菌感染植物组织为研究伴随植物发育的细胞代谢动态提供了一个便利的模型。迄今为止，植物对农杆菌感染的早期和晚期反应在转录组水平上已得到充分表征，而关于伴随的植物代谢变化的信息却很少。因此，在这里我们采用蛋白质组学和代谢组学相结合的方法来研究植物对接种农杆菌培养物的反应所伴随的代谢变化和分子事件。基于获得的蛋白质组学数据集以及代谢物谱分析实验的结果，我们成功地表征了与农杆菌感染相关的代谢变化。观察到的幼苗蛋白质组和代谢组动态清楚地表明，接种后第10天（d.a.i.）能量代谢发生了重排。具体而言，观察到能量代谢从氧化途径转向厌氧途径。这可能是植物对肿瘤诱导的缺氧胁迫的适应性反应的一部分，这很可能涉及糖信号的激活。
