Department of Kinesiology, University of Texas at Arlington, Arlington, Texas.
Delaware Biotechnology Institute, University of Delaware, Newark, Delaware.
Microcirculation. 2019 Nov;26(8):e12579. doi: 10.1111/micc.12579. Epub 2019 Jul 21.
To characterize ossified bone marrow blood vessels and confirm the presence of ossified particles (OSP) in humans and rodents.
Human bone marrow blood vessels were processed for scanning and transmission electron microscopy. Whole blood samples were collected from younger (26-39 years; n = 6) and older (55-63 years; n = 6) volunteers and male Fischer-344 rats (1 month, n = 7; 6 months, n = 7; 12 months, n = 7; 18-months, n = 6; 24 months, n = 8). OSP in the whole blood samples were sorted and imaged with microscopy to determine diameter, circularity, and solidity. Additionally, the chemical composition of OSP was determined via elemental analysis.
SEM revealed two types of ossified bone marrow blood vessels: that is, "transitioning" and "ossified." OSP were adhered to the surface of transitioning vessels and theoretically gain access to and circulate within the blood. The majority of OSP were ≤15 μm in diameter, but many were of sufficient size to serve as emboli (ie, >15 μm).OSP were predominately oblong in shape and several had jagged tips and edges.
We introduce a novel, bone-like blood particle that may be diagnostic of bone marrow blood vessel ossification. Further, OSP may associate with several disease states (eg, atherosclerosis).
对骨髓血管骨化进行特征描述，并确认在人类和啮齿动物中存在骨化颗粒（OSP）。
对人类骨髓血管进行扫描和透射电子显微镜处理。从年龄较小（26-39 岁；n=6）和较大（55-63 岁；n=6）的志愿者以及雄性 Fischer-344 大鼠（1 个月，n=7；6 个月，n=7；12 个月，n=7；18 个月，n=6；24 个月，n=8）采集全血样本。使用显微镜对全血样本中的 OSP 进行分类和成像，以确定直径、圆度和致密度。此外，通过元素分析确定 OSP 的化学成分。
SEM 显示了两种类型的骨化骨髓血管：即“过渡型”和“骨化型”。OSP 附着在过渡型血管的表面，理论上可以进入并在血液中循环。大多数 OSP 的直径≤15 μm，但有许多 OSP 足够大，可以作为栓子（即>15 μm）。OSP 主要呈长形，有几个具有锯齿状的尖端和边缘。
我们介绍了一种新的、类似骨骼的血液颗粒，它可能是骨髓血管骨化的诊断标志物。此外，OSP 可能与几种疾病状态（例如动脉粥样硬化）有关。
