Hirano T, Rabbi M E, Taguchi K, Iwasaki K
Department of Orthopaedic Surgery, Nagasaki University School of Medicine, Japan.
Calcif Tissue Int. 1994 Feb;54(2):160-4. doi: 10.1007/BF00296068.
To investigate the development of the blood vessels forming the secondary ossification center in the femoral heads of growing rats, we observed the specimens of the proximal femurs having vascular casts using both an optical and a scanning electron microscopy (SEM). Histologically, the vascular invasion occurred from the lateral part of the cartilaginous epiphysis, prior to the appearance of the ossification center. The vascular casts were prominent within the cellular spaces consisting of a few hypertrophic chondrocytes. The SEM study suggested that the vascular buds had peculiar structures and invaded the cartilaginous epiphysis by breaking through the cellular spaces. The blood vessels budded out from the arch of the lateral epiphyseal vessels toward the femoral heads. The sizes of the large-caliber vascular buds of about 50 microns in diameter were similar to those of the cellular spaces, and they penetrated into the epiphysis by repeated merging and branching. Many deep impressions were noted on the surface of the vascular buds, and the tips showed the peculiar shape similar to a suction cup.
为了研究正在生长的大鼠股骨头中形成次级骨化中心的血管发育情况，我们使用光学显微镜和扫描电子显微镜（SEM）观察了具有血管铸型的股骨近端标本。组织学上，在骨化中心出现之前，血管从软骨骨骺的外侧部分侵入。血管铸型在由一些肥大软骨细胞组成的细胞间隙内很明显。扫描电子显微镜研究表明，血管芽具有特殊结构，并通过穿透细胞间隙侵入软骨骨骺。血管从外侧骨骺血管弓向股骨头发出芽。直径约50微米的大口径血管芽的大小与细胞间隙相似，它们通过反复合并和分支进入骨骺。在血管芽的表面有许多深凹痕，其尖端呈现出类似于吸盘的特殊形状。
