Ferreirós C M, Criado M T, Sáinz V, Carballo J, del Río C, Suárez B
Dep. Microbiología, Colegio Universitario de La Coruña, Spain.
Ann Inst Pasteur Microbiol (1985). 1986 Jul-Aug;137B(1):37-45. doi: 10.1016/s0769-2609(86)80092-8.
Surface hydrophobicity and adherence to human buccal epithelial cells were evaluated in four Neisseria meningitidis strains. Hydrophobicity was measured by partition into p-xylene in the presence or absence of ammonium sulphate, whereas radioactivity-labelled bacteria were used to estimate the ability to adhere to buccal cells by liquid scintillation techniques. Eight antibiotics were employed to induce modifications in the two parameters in order to estimate their possible relationship. The presence of ammonium sulphate enhanced the partition into p-xylene and hence the hydrophobicity values obtained. There was no correlation between adherence and hydrophobicity (no matter what the method employed to evaluate the latter). The effect of the antibiotics varied as a function of the strain, method and/or parameter tested.
对四株脑膜炎奈瑟菌菌株的表面疏水性及对人颊上皮细胞的黏附性进行了评估。疏水性通过在有或无硫酸铵存在的情况下分配至对二甲苯中进行测量，而放射性标记的细菌则用于通过液体闪烁技术估计黏附于颊细胞的能力。使用了八种抗生素来诱导这两个参数的变化，以评估它们之间可能存在的关系。硫酸铵的存在增强了向对二甲苯中的分配，从而提高了所获得的疏水性值。黏附性与疏水性之间没有相关性（无论采用何种方法评估后者）。抗生素的作用因菌株、方法和/或所测试的参数而异。
