Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2019 Dec 5;223:117263. doi: 10.1016/j.saa.2019.117263. Epub 2019 Jun 10.
Chiral recognition of enantiomers is fundamentally important. In this work, an effective and simple chiral sensing strategy for discrimination of leucine (Leu) enantiomers was constructed. In this chiral sensing, cube-shaped gold nanoparticles (AuNCs) as colorimetric probes are used to recognize Leu enantiomers. L-Leu can induce rapidly the aggregation of AuNCs, leading to change of AuNCs solution from red to colorless, while D-Leu cannot induce the color change. This distinct color changes allow naked-eye to distinguish chiral isomers of Leu. The chiral sensing was applied to measure the enantiometric excess of L-Leu in the whole range (from -100% to 100%). This chiral analysis can be performed by naked eye or simple ultraviolet-visible spectrometer. In addition, the mechanism of chiral recognition has been studied with circular dichroism (CD) spectra, UV-vis absorption spectra and zeta potential. In this method, the chiral recognition is due to the intrinsic chirality of AuNCs, and the AuNCs don't need any chiral labeling or modification. This chiral sensing method is simple, cheap, rapid and easy to operate. Furthermore, this work provides one experimental evidence for intrinsic chirality of nanoparticles, and helps people understand the chirality of nanostructures.
对映异构体的手性识别具有重要的意义。在这项工作中，构建了一种用于识别亮氨酸（Leu）对映异构体的有效且简单的手性传感策略。在这种手性传感中，立方形状的金纳米粒子（AuNCs）作为比色探针用于识别 Leu 对映异构体。L-Leu 可以快速诱导 AuNCs 的聚集，导致 AuNCs 溶液从红色变为无色，而 D-Leu 不能诱导颜色变化。这种明显的颜色变化可以使肉眼区分 Leu 的手性异构体。手性传感用于测量 L-Leu 的对映体过量值（从-100%到 100%）。这种手性分析可以通过肉眼或简单的紫外可见分光光度计进行。此外，通过圆二色（CD）光谱、紫外-可见吸收光谱和动电电势研究了手性识别的机制。在该方法中，手性识别是由于 AuNCs 的固有手性，AuNCs 不需要任何手性标记或修饰。该手性传感方法简单、廉价、快速且易于操作。此外，这项工作为纳米粒子的固有手性提供了一个实验证据，有助于人们理解纳米结构的手性。
