Canada's Michael Smith Genome Sciences Centre, British Columbia Cancer, Vancouver, BC V5Z-4S6, Canada.
Department of Medical Genetics, University of British Columbia, Vancouver, BC V6T-1Z4, Canada.
Genes (Basel). 2019 Jun 26;10(7):486. doi: 10.3390/genes10070486.
The Steller sea lion is the largest member of the Otariidae family and is found in the coastal waters of the northern Pacific Rim. Here, we present the Steller sea lion genome, determined through DNA sequencing approaches that utilized microfluidic partitioning library construction, as well as nanopore technologies. These methods constructed a highly contiguous assembly with a scaffold N50 length of over 14 megabases, a contig N50 length of over 242 kilobases and a total length of 2.404 gigabases. As a measure of completeness, 95.1% of 4104 highly conserved mammalian genes were found to be complete within the assembly. Further annotation identified 19,668 protein coding genes. The assembled genome sequence and underlying sequence data can be found at the National Center for Biotechnology Information (NCBI) under the BioProject accession number PRJNA475770.
拟态海豹是石斑海豹科中最大的成员，分布于北太平洋沿岸水域。在这里，我们展示了拟态海豹基因组，该基因组通过 DNA 测序方法确定，这些方法利用了微流控分区文库构建以及纳米孔技术。这些方法构建了一个高度连续的组装体，支架 N50 长度超过 1400 万碱基对，连续体 N50 长度超过 242000 碱基对，总长度为 24.04 亿碱基对。作为完整性的衡量标准，在组装体中发现了 4104 个高度保守的哺乳动物基因中有 95.1%是完整的。进一步注释确定了 19668 个蛋白质编码基因。组装的基因组序列和基础序列数据可在国家生物技术信息中心 (NCBI) 下的生物项目访问号 PRJNA475770 中找到。
