Department of Periodontics, College of Dental Sciences, Davangere, India.
Indian J Med Res. 2019 Mar;149(3):364-368. doi: 10.4103/ijmr.IJMR_1821_16.
BACKGROUND & OBJECTIVES: : There is a paucity of information on association between dental fluorosis, osteoporosis and periodontitis. The aim of this pilot study was to evaluate oestrogen receptor (ER).
RSA 1: gene polymorphism in osteoporosis periodontitis patients with and without dental fluorosis.
: Twenty one primary osteoporotic patients suffering from periodontitis with dental fluorosis and 20 primary osteoporotic patients suffering from periodontitis without dental fluorosis participated in this study. Periodontitis was diagnosed based on age, gender T-scores using clinical parameters such as plaque scores, gingival bleeding scores and probing pocket depth, clinical attachment level (CAL) and severity of dental fluorosis. DNA was genotyped at the RsaI RFLP (in exon 5) inside the ER gene to study ER Rsa I gene polymorphism in osteoporosis periodontitis patients with and without dental fluorosis.
: Patients with dental fluorosis had higher degree of osteoporosis than those without fluorosis. CAL was significantly higher (P <0.05) in those with dental fluorosis compared with those without. Rr heterozygote (21.95%) was observed in patients without fluorosis whereas RR mutant homozygote was absent in both the groups. Rr wild homozygote type was seen more in the patients with fluorosis (51.21%). Significant differences were found in distribution of these genotypes between patients with and without dental fluorosis.
INTERPRETATION & CONCLUSIONS: : This preliminary study showed the presence of ER I gene polymorphism in osteoporosis periodontitis patients without dental fluorosis. Further studies with large sample size are needed to confirm the association shown in this preliminary study.
关于氟斑牙、骨质疏松症和牙周炎之间的关联，信息匮乏。本初步研究旨在评估雌激素受体（ER）。
RSA1：在患有氟斑牙和不患有氟斑牙的骨质疏松症-牙周炎患者中的基因多态性。
21 名原发性骨质疏松症合并牙周炎且患有氟斑牙的患者和 20 名原发性骨质疏松症合并牙周炎但不患有氟斑牙的患者参与了本研究。牙周炎的诊断依据为年龄、性别 T 分数以及临床参数，如菌斑评分、牙龈出血评分和探诊袋深度、临床附着水平（CAL）和氟斑牙的严重程度。在 ER 基因的 RsaI RFLP（外显子 5）处对 DNA 进行基因分型，以研究患有和不患有氟斑牙的骨质疏松症-牙周炎患者中的 ER Rsa I 基因多态性。
患有氟斑牙的患者骨质疏松程度高于不患有氟斑牙的患者。患有氟斑牙的患者 CAL 显著升高（P <0.05）。在不患有氟斑牙的患者中观察到 rr 杂合子（21.95%），而在两组患者中均不存在 RR 突变纯合子。rr 野生纯合子类型在患有氟斑牙的患者中更为常见（51.21%）。在患有和不患有氟斑牙的患者中，这些基因型的分布存在显著差异。
本初步研究显示，在不患有氟斑牙的骨质疏松症-牙周炎患者中存在 ER I 基因多态性。需要进一步进行更大样本量的研究以证实本初步研究中显示的相关性。
