Tsutsumi S, Ishibashi K, Miyai K, Nagase S, Ito M, Amino N, Endo Y
Eiken Immunochemical Laboratories, Tochigi, Japan.
Clin Chim Acta. 1987 Dec;170(2-3):315-22. doi: 10.1016/0009-8981(87)90142-2.
We describe a double-antibody solid phase radioimmunoassay for free thyroxine (FT4) in serum with use of 125I-labelled thyroxine-human chorionic gonadotropin conjugate. Since the labelled conjugate does not bind to thyroxine binding globulin (TBG) and albumin because of its large molecular weight, the method is uninfluenced by TBG or albumin. The measurable range of FT4 in serum was 2.0 to 128 ng/l. The mean coefficients of variation within and between assays were 4.6-8.6% and 6.3-11.6%, respectively. The FT4 values determined by the proposed method correlated well with those determined by commercial radioimmunoassay in subjects with normal albumin concentration (r = 0.98). The FT4 concentrations in serum as determined by this method were 9 to 17 ng/l for healthy adult subjects; high for patients with hyperthyroidism; low for patients with hypothyroidism; and within normal limits for pregnant women, and patients with high or low concentrations of thyroxine-binding globulin.
我们描述了一种用于血清中游离甲状腺素（FT4）的双抗体固相放射免疫分析法，该方法使用了125I标记的甲状腺素-人绒毛膜促性腺激素偶联物。由于标记的偶联物分子量较大，不与甲状腺素结合球蛋白（TBG）和白蛋白结合，因此该方法不受TBG或白蛋白的影响。血清中FT4的可测量范围为2.0至128 ng/l。测定内和测定间的平均变异系数分别为4.6 - 8.6%和6.3 - 11.6%。在白蛋白浓度正常的受试者中，用本方法测定的FT4值与用商业放射免疫分析法测定的值相关性良好（r = 0.98）。用该方法测定，健康成年受试者血清中的FT4浓度为9至17 ng/l；甲状腺功能亢进患者的FT4浓度较高；甲状腺功能减退患者的FT4浓度较低；孕妇以及甲状腺素结合球蛋白浓度高或低的患者的FT4浓度在正常范围内。
