Ito M, Miyai K, Doi K, Mizuta H, Amino N
Clin Chem. 1984 Oct;30(10):1682-5.
We describe a double-antibody enzyme immunoassay for free thyroxin (FT4) in serum with use of beta-D-galactosidase conjugated to thyroxin. The method is uninfluenced by thyroxin-binding globulin or albumin. Values for FT4 so determined correlated well with those determined by radioimmunoassay (r = 0.98) and equilibrium dialysis (r = 0.89). The mean variability (CV) within and between assays was 7.4% and 7.6%, respectively. The measurable range of FT4 in serum was 2.8 to 109 ng/L. The FT4 concentrations in serum as determined by this method were 8.4 to 15.5 ng/L for 26 normal adult subjects; 26 to greater than 109 ng/L for 10 patients with hyperthyroidism; less than 2.8 to 8.0 ng/L for seven patients with hypothyroidism; 7.3 to 15.8 ng/L for eight pregnant women; and 12.2 and 13.5 ng/L for two patients with low concentrations of thyroxin-binding globulin.
我们描述了一种使用与甲状腺素偶联的β-D-半乳糖苷酶对血清中游离甲状腺素（FT4）进行双抗体酶免疫测定的方法。该方法不受甲状腺素结合球蛋白或白蛋白的影响。如此测定的FT4值与通过放射免疫测定法（r = 0.98）和平衡透析法（r = 0.89）测定的值相关性良好。测定内和测定间的平均变异系数（CV）分别为7.4%和7.6%。血清中FT4的可测量范围为2.8至109 ng/L。通过该方法测定，26名正常成年受试者血清中的FT4浓度为8.4至15.5 ng/L；10名甲状腺功能亢进患者为26至大于109 ng/L；7名甲状腺功能减退患者为低于2.8至8.0 ng/L；8名孕妇为7.3至15.8 ng/L；2名甲状腺素结合球蛋白浓度低的患者为12.2和13.5 ng/L。
