血管加压素对小鼠3T3细胞生长的刺激作用。
Vasopressin stimulation of mouse 3T3 cell growth.
作者信息
Rozengurt E, Legg A, Pettican P
出版信息
Proc Natl Acad Sci U S A. 1979 Mar;76(3):1284-7. doi: 10.1073/pnas.76.3.1284.
Abstract
Vasopressin is shown to be a potent mitogen for Swiss 3T3 cells. The hormone (1--10 ng/ml) causes a striking shift of the dose--response curve for the effect of serum on thymidine incorporation by cultures of 3T3 cells arrested in the G1/G0 phase of the cell cycle. In the absence of added serum, the effect of vasopressin on DNA synthesis is greatly potentiated by insulin, epidermal growth factor, and a factor isolated from medium conditioned by simian virus 40-infected baby hamster kidney cells. The mitogenic effect of vasopressin is dependent on time and hormone concentration. In the presence of insulin, the half-maximal effect elicited by the peptide is obtained at 0.6 ng/ml. [Arg]Vasopressin and [Lys]vasopressin are equally potent. The vasopressins are 10(3)-fold more potent than oxytocin. In the presence of a low (2.5%) concentration of serum, vasopressins stimulate cell proliferation.
摘要
血管加压素被证明是瑞士3T3细胞的一种强效促有丝分裂原。该激素（1 - 10纳克/毫升）会使血清对处于细胞周期G1/G0期的3T3细胞培养物中胸腺嘧啶核苷掺入的剂量 - 反应曲线发生显著偏移。在不添加血清的情况下，胰岛素、表皮生长因子以及从感染猿猴病毒40的幼仓鼠肾细胞条件培养基中分离出的一种因子可极大增强血管加压素对DNA合成的作用。血管加压素的促有丝分裂作用取决于时间和激素浓度。在有胰岛素存在的情况下，该肽引发的半最大效应在0.6纳克/毫升时获得。[精氨酸]血管加压素和[赖氨酸]血管加压素的效力相同。血管加压素的效力比催产素强1000倍。在低浓度（2.5%）血清存在的情况下，血管加压素会刺激细胞增殖。
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本文引用的文献
1
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J Cell Biol. 1963 May;17(2):299-313. doi: 10.1083/jcb.17.2.299.
2
The production of markers by bronchogenic carcinoma: a review.支气管源性癌标志物的产生：综述
Semin Oncol. 1974 Sep;1(3):235-44.
3
Control of growth of mammalian cells in cell culture.细胞培养中哺乳动物细胞生长的控制
Nature. 1975 Dec 11;258(5535):487-90. doi: 10.1038/258487a0.
4
Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells.血清能迅速刺激静止的3T3细胞中哇巴因敏感的86-RB+内流。
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4492-5. doi: 10.1073/pnas.72.11.4492.
5
A highly specific antiserum for arginine vasopressin.一种针对精氨酸加压素的高度特异性抗血清。
Endocrinology. 1977 Nov;101(5):1421-35. doi: 10.1210/endo-101-5-1421.
6
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J Endocrinol. 1977 Jan;72(1):5-16. doi: 10.1677/joe.0.0720005.
7
Growth factors in mammalian cell culture.哺乳动物细胞培养中的生长因子。
Annu Rev Biochem. 1976;45:531-58. doi: 10.1146/annurev.bi.45.070176.002531.
8
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J Cell Physiol. 1978 Dec;97(3 Pt 2 Suppl 1):441-9. doi: 10.1002/jcp.1040970319.
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10
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