Johnson D H, Edström J E, Burnett J B, Friedman T B
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
Gene. 1987;59(1):77-86. doi: 10.1016/0378-1119(87)90268-x.
The Aprt locus of Drosophila melanogaster encodes the structural gene for adenine phosphoribosyltransferase (APRT). DNA cloned from microdissected salivary gland polytene chromosome region 62B7-12 was used in conjunction with chromosome walking and hybrid selection of mRNA to isolate the Aprt gene. Aprt lies at cytogenetic position 62B9 and is closely flanked by other genes of unknown function. Nucleotide sequencing shows that four APRT cDNAs have a common 5' terminus with an apparent cap consensus sequence but two different 3' sites of polyadenylation. The distribution of conserved amino acid sequences in APRT from vertebrates, insects and bacteria suggests that they may have shared a common ancestral gene for this ubiquitous enzyme.
黑腹果蝇的Aprt基因座编码腺嘌呤磷酸核糖转移酶（APRT）的结构基因。从显微切割的唾液腺多线染色体区域62B7 - 12克隆的DNA与染色体步移和mRNA的杂交筛选相结合，用于分离Aprt基因。Aprt位于细胞遗传学位置62B9，其两侧紧密排列着其他功能未知的基因。核苷酸测序表明，四个APRT cDNA具有一个带有明显帽状共有序列的共同5'末端，但有两个不同的3'聚腺苷酸化位点。脊椎动物、昆虫和细菌的APRT中保守氨基酸序列的分布表明，它们可能为这种普遍存在的酶共享一个共同的祖先基因。
