Wallrath L L, Burnett J B, Friedman T B
Graduate Program in Genetics, Michigan State University, East Lansing 48824.
Mol Cell Biol. 1990 Oct;10(10):5114-27. doi: 10.1128/mcb.10.10.5114-5127.1990.
The urate oxidase (UO) gene of Drosophila melanogaster is expressed during the third-instar larval and adult stages, exclusively within a subset of cells of the Malpighian tubules. The UO gene contains a 69-base-pair intron and encodes mature mRNAs of 1,224, 1,227, and 1,244 nucleotides, depending on the site of 3' endonucleolytic cleavage prior to polyadenylation. A direct repeat, 5'-AAGTGAGAGTGAT-3', is the proposed cis-regulatory element involved in 20-hydroxyecdysone repression of the UO gene. The deduced amino acid sequences of UO of D. melanogaster, rat, mouse, and pig and uricase II of soybean show 32 to 38% identity, with 22% of amino acid residues identical in all species. With use of P-element-mediated germ line transformation, 826 base pairs 5' and approximately 1,200 base pairs 3' of the D. melanogaster UO transcribed region contain all of the cis elements allowing for appropriate temporal regulation and Malpighian tubule-specific expression of the UO gene.
黑腹果蝇的尿酸氧化酶（UO）基因在三龄幼虫和成虫阶段表达，且仅在马氏管的一部分细胞中表达。UO基因包含一个69个碱基对的内含子，根据聚腺苷酸化前3' 核酸内切酶切割的位点，编码1224、1227和1244个核苷酸的成熟mRNA。一个直接重复序列5'-AAGTGAGAGTGAT-3' 是推测的参与20-羟基蜕皮激素对UO基因抑制作用的顺式调控元件。黑腹果蝇、大鼠、小鼠和猪的UO以及大豆的尿酸酶II的推导氨基酸序列显示出32%至38%的同一性，所有物种中有22%的氨基酸残基相同。利用P元素介导的种系转化，黑腹果蝇UO转录区域5' 端的826个碱基对和3' 端大约1200个碱基对包含了所有允许UO基因进行适当的时间调控和马氏管特异性表达的顺式元件。
