人腺嘌呤磷酸核糖转移酶基因（APRT）位于触珠蛋白（HP）和16号染色体脆性位点（fra(16)(q23)，即FRA16D）位点远端的一个新位置。

A new location for the human adenine phosphoribosyltransferase gene (APRT) distal to the haptoglobin (HP) and fra(16)(q23)(FRA16D) loci.

作者信息

Fratini A, Simmers R N, Callen D F, Hyland V J, Tischfield J A, Stambrook P J, Sutherland G R

出版信息

Cytogenet Cell Genet. 1986;43(1-2):10-3. doi: 10.1159/000132291.

Abstract

The human adenine phosphoribosyltransferase gene (APRT) was mapped with respect to the haptoglobin gene (HP) and the fragile site at 16q23.2 (FRA16D). A subclone of APRT and a cDNA clone of HP were used for molecular hybridization to DNA from mouse-human hybrid cell lines containing specific chromosome 16 translocations. The APRT subclone was used for in situ hybridization to chromosomes expressing FRA16D. APRT was found to be distal to HP and FRA16D and was localized at 16q24, making the gene order cen-FRA16B-HP-FRA16D-APRT-qter.

摘要

人类腺嘌呤磷酸核糖转移酶基因（APRT）相对于触珠蛋白基因（HP）和位于16q23.2的脆性位点（FRA16D）进行了定位。APRT的一个亚克隆和HP的一个cDNA克隆用于与来自含有特定16号染色体易位的小鼠-人杂交细胞系的DNA进行分子杂交。APRT亚克隆用于对表达FRA16D的染色体进行原位杂交。发现APRT位于HP和FRA16D的远端，定位于16q24，基因顺序为cen-FRA16B-HP-FRA16D-APRT-qter。