Park Jin-Ho, Yeom Gyuho, Hong Donggu, Jo Eun-Jung, Park Chin-Ju, Kim Min-Gon
Department of Chemistry, Gwangju Institute of Science and Technology (GIST), 123 Cheomdangwagi-ro, Buk-Gu, Gwangju 61005, Korea.
Center for Systems Biology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.
Sensors (Basel). 2019 Jun 27;19(13):2857. doi: 10.3390/s19132857.
A simple and rapid As detection method using 3-nitro-L-tyrosine (N-Tyr) is reported. We discovered the specific property of N-Tyr, which specifically chelates As. The reaction between As and N-Tyr induces a prompt color change to vivid yellow, concomitantly increasing the absorbance at 430 nm. The selectivity for As is confirmed by competitive binding experiments with various metal ions (Hg, Pb, Cd, Cr, Mg, Ni, Cu, Fe, Ca, Zn, and Mn). Also, the N-Tyr binding site, binding affinity, and As/N-Tyr reaction stoichiometry are investigated. The specific reaction is utilized to design a sensor that enables the quantitative detection of As in the 0.1-100 μM range with good linearity (R = 0.995). Furthermore, the method's applicability for the analysis of real samples, e.g., tap and river water, is successfully confirmed, with good recoveries (94.32-109.15%) using As-spiked real water samples. We believe that our discovering and its application for As analysis can be effectively utilized in environmental analyses such as those conducted in water management facilities, with simplicity, rapidity, and ease.
报道了一种使用3-硝基-L-酪氨酸（N-Tyr）的简单快速的砷检测方法。我们发现了N-Tyr的特殊性质，即它能特异性螯合砷。砷与N-Tyr之间的反应会迅速使颜色变为鲜黄色，同时在430nm处的吸光度增加。通过与各种金属离子（汞、铅、镉、铬、镁、镍、铜、铁、钙、锌和锰）的竞争性结合实验证实了对砷的选择性。此外，还研究了N-Tyr的结合位点、结合亲和力以及砷/N-Tyr反应化学计量比。利用这种特异性反应设计了一种传感器，能够在0.1-100μM范围内对砷进行定量检测，线性良好（R = 0.995）。此外，该方法对实际样品（如自来水和河水）分析的适用性也得到了成功证实，使用加标实际水样时回收率良好（94.32-109.15%）。我们相信，我们的发现及其在砷分析中的应用可以简单、快速且轻松地有效地应用于环境分析，如水处理设施中的分析。
