西班牙北部淋球菌感染的流行病学监测研究。

Epidemiological surveillance study of gonococcal infection in Northern Spain.

机构信息

Departamento de Inmunología, Microbiología y Parasitología (Facultad de Medicina), Universidad del País Vasco, Leioa, Vizcaya, Spain.

Servicio de Microbiología Clínica y Control de Infección, Hospital Universitario Basurto, Bilbao, Vizcaya, Spain.

出版信息

Enferm Infecc Microbiol Clin (Engl Ed). 2020 Feb;38(2):59-64. doi: 10.1016/j.eimc.2019.05.002. Epub 2019 Jun 27.

DOI:10.1016/j.eimc.2019.05.002

PMID:31255376

Abstract

BACKGROUND

Treatment of gonorrhoea is threatened by antimicrobial resistance, and decreased susceptibility to recommended therapies is emerging. Thus, gonococcal infection (GI) is becoming a public health problem. The objectives of the present study were to monitor the antimicrobial sensitivity in Neisseria gonorrhoeae (NG) during 2011-2015 and to study their genogroups.

METHODS

Antimicrobial susceptibility was studied by disc diffusion, in addition to the agar dilution method for cefixime and ceftriaxone and the Etest® for azithromycin. Genotyping was performed by the NG multi-antigen sequence typing (NG-MAST) method. Genogroups of closely related sequence types (STs) were defined.

RESULTS

All the strains were susceptible to cefixime, ceftriaxone and gentamicin and 1.8% of the strains were resistant to azithromycin. A total of 531 STs and 6 genotypes (Gs) were identified during 2012-2015 period. G2992 was the largest and was associated with resistance to azithromycin, and with men who have sex with men (MSM), alongside G2400. G1407 and G2400 strains were related to high minimum inhibitory concentration (MICs) to cefixime and G1407 also to ceftriaxone. For the first time, G1861 and G2018 were described and associated with ciprofloxacin resistance and G2018 also with high MICs to ceftriaxone.

CONCLUSION

Molecular typing is a useful tool to predict antimicrobial resistance. These results show the need to develop novel antimicrobials or to design new antimicrobial therapies based on drugs that show their efficacy against GI. This also highlights the importance of developing sexually transmitted infection (STI) surveillance in homosexual populations.

摘要

背景

淋病的治疗受到抗菌药物耐药性的威胁，而对抗推荐疗法的敏感性降低正在出现。因此，淋球菌感染（GI）正在成为一个公共卫生问题。本研究的目的是监测 2011-2015 年淋病奈瑟菌（NG）的抗菌敏感性，并研究其基因群。

方法

通过纸片扩散法研究抗菌敏感性，此外还通过琼脂稀释法研究头孢克肟和头孢曲松以及 Etest®法研究阿奇霉素的敏感性。通过 NG 多抗原序列分型（NG-MAST）方法进行基因分型。定义了密切相关序列型（ST）的基因群。

结果

所有菌株均对头孢克肟、头孢曲松和庆大霉素敏感，1.8%的菌株对阿奇霉素耐药。在 2012-2015 年期间共鉴定出 531 个 ST 和 6 个基因型（Gs）。G2992 是最大的基因群，与耐阿奇霉素以及与男男性行为者（MSM）相关，与 G2400 相关。G1407 和 G2400 菌株与头孢克肟的最低抑菌浓度（MIC）较高有关，而 G1407 也与头孢曲松有关。首次描述了 G1861 和 G2018，并与环丙沙星耐药有关，而 G2018 也与头孢曲松的 MIC 较高有关。